Western blot analysis of mRNA capping enzyme using anti-mRNA capping enzyme monoclonal antibody (MA5-17255) on (Lane 1) Bosc23 Cell lysate, (Lane 2) Bosc23 Cell lysate transfected with Myc/His/Tau, (Lane 3) Bosc23 Cell lysateMyc/His/PTPRR and (Lane 4) Bosc23 Cell lysate GFP-Ub-2.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is Bosc23 cell lysate transfected with Myc/His/mRNA-capping enzyme.
This protein is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.