|Tested species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to human HDAC7 (aa 400-500)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5-10 µg/ml|
|Immunofluorescence (IF)||5-10 µg/ml|
|Immunohistochemistry (IHC)||2-5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-5 µg/ml|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody may detect multiple isoforms of HDAC7.
Histone deacetylase 7 is a thymus-specific, intracellular transcription factor belonging to the class IIa histone deacetylase family. Reports suggest that dephosphorylation and nuclear localization of HDAC-7 is promoted by Myosin phosphatase, thus regulating the repression of Nur77 and inhibition of apoptosis in CD4+CD8+ double-positive thymocytes. HDAC-7 plays a specific role in maintaining vascular integrity by repressing the expression of matrix metalloproteinase (MMP) 10. It promotes repression mediated by transcriptional corepressor NCOR2 and is an efficient corepressor of the androgen receptor (AR). It is also responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Chromatin packaging and remodeling Protein metabolism and modification Protein modification Cell cycle Cell cycle control