MA1-20354 detects HDAC1 in human and mouse samples. Expected to cross react with rat due to sequence homology.
MA1-20354 has been successfully used in ELISA, immunoprecipitation and Western blot procedures. MA1-20354 detects a band ~65 kDa by Western blot. Possible positive controls for this product are HeLa cell whole cell lysate.
The MA1-20354 immunogen is a synthetic peptide corresponding to residues K(466) E E K P E A K G V K E E V K L A(482) of human HDAC1.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp686H12203; GON-10; HD1; Histone Deacetylase 1; reduced potassium dependency, yeast homolog-like 1; RPD3; RPD3L1
Gene Aliases: GON-10; HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1