Invitrogen

HDAC3 Recombinant Rabbit Monoclonal Antibody (SC55-02)

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HDAC3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of HDAC3 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HDAC3 Recombinant Monoclonal Antibody (Product # MA5-41156) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

HDAC3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of HDAC3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HDAC3 Recombinant Monoclonal Antibody (Product # MA5-41156) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC3 in paraffin-embedded human breast carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with HDAC3 Recombinant Monoclonal Antibody (Product # MA5-41156) at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC3 in paraffin-embedded human tonsil tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with HDAC3 Recombinant Monoclonal Antibody (Product # MA5-41156) at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

HDAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC3 in paraffin-embedded mouse skin tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with HDAC3 Recombinant Monoclonal Antibody (Product # MA5-41156) at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

HDAC3 Antibody in Western Blot (WB) — Western Blot analysis of HDAC3 using HDAC3 Recombinant Monoclonal Antibody (Product # MA5-41156) at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: HL-60 cell lysate, Lane 2: K562 cell lysate.

Product Details

MA5-41156

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	SC55-02
Immunogen	Synthetic peptide within C-terminal human HDAC3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; RPD3-2; SMAP45

Gene Aliases: AW537363; HD3; HDAC3; RPD3; RPD3-2

UniProt ID: (Human) O15379, (Rat) Q6P6W3

Entrez Gene ID: (Human) 8841, (Mouse) 15183, (Rat) 84578

negative regulation of transcription from RNA polymerase II promoter positive regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated protein deacetylation regulation of mitotic cell cycle chromatin modification histone deacetylation regulation of protein stability positive regulation of TOR signaling negative regulation of interleukin-1 production negative regulation of tumor necrosis factor production circadian regulation of gene expression regulation of multicellular organism growth positive regulation of transcription factor import into nucleus negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of JNK cascade negative regulation of protein export from nucleus spindle assembly cellular response to fluid shear stress positive regulation of type B pancreatic cell apoptotic process negative regulation of cardiac muscle cell differentiation negative regulation of myotube differentiation response to nutrient levels response to drug histone H3-K9 modification histone H3 deacetylation histone H4 deacetylation cellular response to mechanical stimulus cellular response to parathyroid hormone stimulus response to dexamethasone circadian rhythm negative regulation of apoptotic process cellular lipid metabolic process

Process(es)

chromatin binding transcription corepressor activity histone deacetylase activity protein binding transcription factor binding enzyme binding cyclin binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity histone deacetylase binding NF-kappaB binding DNA binding hydrolase activity histone deacetylase activity (H3-K14 specific) chromatin DNA binding histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) GTPase binding NAD-dependent histone deacetylase activity (H3-K18 specific)