Invitrogen

HDAC6 Recombinant Rabbit Monoclonal Antibody (JJ09-09)

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Antibody Testing Data (7)

HDAC6 Antibody in Western Blot (WB)

FIGURE: 1 / 7

HDAC6 Antibody (MA5-32468) in WB

Western blot was performed using Anti-HDAC6 Recombinant Rabbit Monoclonal Antibody (JJ09-09) (Product # MA5-32468) and a 160kDa band corresponding to HDAC6 was observed in the cell lines tested. Whole cell extracts of A549 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), HT-29 (Lane 4) and MDCK (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed ... View More

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HDAC6 Antibody in Western Blot (WB) — Western blot was performed using Anti-HDAC6 Recombinant Rabbit Monoclonal Antibody (JJ09-09) (Product # MA5-32468) and a 160kDa band corresponding to HDAC6 was observed in the cell lines tested. Whole cell extracts of A549 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), HT-29 (Lane 4) and MDCK (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HDAC6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of HDAC6 in HepG2 cells using a HDAC6 Monoclonal antibody (Product # MA5-32468) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HDAC6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of HDAC6 in Hela cells using a HDAC6 Monoclonal antibody (Product # MA5-32468) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HDAC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC6 of paraffin-embedded Human kidney tissue using a HDAC6 Monoclonal antibody (Product #MA5-32468). Counter stained with hematoxylin.

HDAC6 Antibody in Western Blot (WB) — Knockdown of HDAC6 was achieved by transfecting A549 with HDAC6 specific siRNAs (Silencer® select Product # s19459). Western blot analysis (Fig. a) was performed using whole cell extracts from the HDAC6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HDAC6 Recombinant Rabbit Monoclonal Antibody (JJ09-09) (Product # MA5-32468, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HDAC6.

HDAC6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of HDAC6 in 293 cells using a HDAC6 Monoclonal antibody (Product # MA5-32468) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HDAC6 Antibody in Western Blot (WB) — Western blot analysis of HDAC6 in Jurkat cell lysates using a HDAC6 Monoclonal antibody (Product # MA5-32468) at a dilution of 1:1,000.

Product Details

MA5-32468

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Dog, Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ09-09

Immunogen

Synthetic peptide within Human HDAC6 aa 1-50

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809745

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ16239; HD6; HDA6; Histone deacetylase 6; protein phosphatase 1, regulatory subunit 90; Tubulin-lysine deacetylase HDAC6

Gene Aliases: CPBHM; HD6; HDAC6; JM21; KIAA0901; PPP1R90

UniProt ID: (Human) Q9UBN7

Entrez Gene ID: (Dog) 480907, (Human) 10013

Molecular Function: chromatin/chromatin-binding, or -regulatory protein histone modifying enzyme

protein polyubiquitination transcription, DNA-templated protein deacetylation misfolded or incompletely synthesized protein catabolic process intracellular protein transport organelle organization negative regulation of microtubule depolymerization response to toxic substance positive regulation of signal transduction response to organic substance regulation of receptor activity regulation of autophagy positive regulation of epithelial cell migration negative regulation of hydrogen peroxide metabolic process positive regulation of receptor biosynthetic process macroautophagy histone deacetylation regulation of protein stability lysosome localization peptidyl-lysine deacetylation cellular response to topologically incorrect protein regulation of gene expression, epigenetic ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway protein complex disassembly negative regulation of protein complex disassembly regulation of fat cell differentiation negative regulation of proteolysis negative regulation of transcription, DNA-templated negative regulation of oxidoreductase activity mitochondrion localization response to misfolded protein regulation of microtubule-based movement regulation of androgen receptor signaling pathway regulation of establishment of protein localization cellular response to hydrogen peroxide aggresome assembly polyubiquitinated misfolded protein transport Hsp90 deacetylation response to growth factor histone H3 deacetylation cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly tubulin deacetylation mitophagy in response to mitochondrial depolarization positive regulation of hydrogen peroxide-mediated programmed cell death regulation of mitophagy

Process(es)

core promoter binding actin binding histone deacetylase activity protein binding beta-catenin binding microtubule binding zinc ion binding enzyme binding polyubiquitin binding ubiquitin protein ligase binding NAD-dependent histone deacetylase activity (H3-K14 specific) histone deacetylase binding tubulin deacetylase activity alpha-tubulin binding tau protein binding beta-tubulin binding misfolded protein binding Hsp90 protein binding dynein complex binding