OriGene

HDAC6 Monoclonal Antibody (OTI4C5), TrueMAB™

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HDAC6 Antibody in Immunofluorescence (IF) — Anti-HDAC6 mouse monoclonal antibody (TA502030) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6(RC209649).

HDAC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

HDAC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human liver tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

HDAC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human colon tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

HDAC6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

HDAC6 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (RC209649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6. Positive lysates LY401822 (100 µg) and LC401822 (20 µg) can be purchased separately from OriGene.

HDAC6 Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.

HDAC6 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC209649 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody (TA502030), and then analyzed by flow cytometry.

HDAC6 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells, using anti-HDAC6 antibody (TA502030), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

HDAC6 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Hela cells, using anti-HDAC6 antibody (TA502030), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

-

Immunocytochemistry (ICC)

1:100

-

Immunofluorescence (IF)

1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:150

-

Western Blot (WB)

1:1000-1:2000

-

Product Specifications

Species Reactivity

Dog, Human, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4C5

Immunogen

Full length human recombinant protein of human HDAC6 produced in HEK293T cell.

Conjugate

Unconjugated

Form

liquid

Concentration

0.84 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ16239; HD6; HDA6; Histone deacetylase 6; protein phosphatase 1, regulatory subunit 90

Gene Aliases: CPBHM; HD6; HDAC6; JM21; KIAA0901; PPP1R90

UniProt ID: (Human) Q9UBN7

Entrez Gene ID: (Dog) 480907, (Human) 10013, (Rat) 84581

Molecular Function: deacetylase hydrolase nucleic acid binding oxidoreductase reductase

protein polyubiquitination transcription, DNA-templated protein deacetylation misfolded or incompletely synthesized protein catabolic process intracellular protein transport organelle organization negative regulation of microtubule depolymerization response to toxic substance positive regulation of signal transduction response to organic substance regulation of receptor activity regulation of autophagy positive regulation of epithelial cell migration negative regulation of hydrogen peroxide metabolic process positive regulation of receptor biosynthetic process macroautophagy histone deacetylation regulation of protein stability lysosome localization peptidyl-lysine deacetylation cellular response to topologically incorrect protein regulation of gene expression, epigenetic ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway protein complex disassembly negative regulation of protein complex disassembly regulation of fat cell differentiation negative regulation of proteolysis negative regulation of transcription, DNA-templated negative regulation of oxidoreductase activity mitochondrion localization response to misfolded protein regulation of microtubule-based movement regulation of androgen receptor signaling pathway regulation of establishment of protein localization cellular response to hydrogen peroxide aggresome assembly polyubiquitinated misfolded protein transport Hsp90 deacetylation response to growth factor histone H3 deacetylation cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly tubulin deacetylation mitophagy in response to mitochondrial depolarization positive regulation of hydrogen peroxide-mediated programmed cell death regulation of mitophagy regulation of protein phosphorylation response to amphetamine ubiquitin-dependent protein catabolic process metabolic process positive regulation of cell proliferation regulation of mitochondrion organization negative regulation of neuron projection development axonal transport of mitochondrion neuron differentiation cellular response to oxidative stress response to immobilization stress negative regulation of axon extension involved in axon guidance positive regulation of dendrite morphogenesis response to corticosterone positive regulation of synaptic transmission, glutamatergic regulation of microtubule cytoskeleton organization cellular response to parathyroid hormone stimulus response to dexamethasone positive regulation of tubulin deacetylation negative regulation of protein acetylation positive regulation of oxidative stress-induced neuron death

Process(es)

core promoter binding actin binding histone deacetylase activity protein binding beta-catenin binding microtubule binding zinc ion binding enzyme binding polyubiquitin binding ubiquitin protein ligase binding NAD-dependent histone deacetylase activity (H3-K14 specific) histone deacetylase binding tubulin deacetylase activity alpha-tubulin binding tau protein binding beta-tubulin binding misfolded protein binding Hsp90 protein binding dynein complex binding hydrolase activity ubiquitin binding