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|Tested species reactivity||Eubacteria, Hamster, Human, Mouse, Primate, Pig, Rabbit, Rat|
|Published species reactivity||Rat, Primate, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||N-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12745 targets HDJ-2/DNAJ in FACS, IF, IHC (P), IP, and WB applications and shows reactivity with Bacteria, Hamster, Human, mouse, Non-human primate, Porcine, Rabbit, and Rat samples.
The MA5-12745 immunogen is n-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein.
It is a novel human homolog of E. coli's DNAJ protein from heat shock protein 40 family. Hsp40 is involved in chaperonic protein folding HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane HDJ-2 has a prenylated C-terminus and is not inducible upon heat shock.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ultrasound safety with midfrequency transcranial sonothrombolysis: preliminary study on normal macaca monkey brain.
MA5-12745 was used in immunohistochemistry to study the safety of midfrequency ultrasound used for thrombolysis in acute ischemic stroke
|Shimizu J,Fukuda T,Abe T,Ogihara M,Kubota J,Sasaki A,Azuma T,Sasaki K,Shimizu K,Oishi T,Umemura S,Furuhata H||Ultrasound in medicine & biology (38:1040)||2012|
A potent HIV protease inhibitor, darunavir, does not inhibit ZMPSTE24 or lead to an accumulation of farnesyl-prelamin A in cells.
MA5-12745 was used in western blot to investigate the effect of darunavir on prelamin A accumulation
|Coffinier C,Hudon SE,Lee R,Farber EA,Nobumori C,Miner JH,Andres DA,Spielmann HP,Hrycyna CA,Fong LG,Young SG||The Journal of biological chemistry (283:9797)||2008|
Inhibition of HMGcoA reductase by atorvastatin prevents and reverses MYC-induced lymphomagenesis.
MA5-12745 was used in western blot to study the mechanism by which atorvastatin inhibition of HMG-CoA reductase prevents and reverses MYC-induced lymphomagenesis
|Shachaf CM,Perez OD,Youssef S,Fan AC,Elchuri S,Goldstein MJ,Shirer AE,Sharpe O,Chen J,Mitchell DJ,Chang M,Nolan GP,Steinman L,Felsher DW||Blood (110:2674)||2007|
HIV protease inhibitors block the zinc metalloproteinase ZMPSTE24 and lead to an accumulation of prelamin A in cells.
MA5-12745 was used in western blot to examine the mechanism for prelamin A accumulation by the treatment of HIV protease inhibitors
|Coffinier C,Hudon SE,Farber EA,Chang SY,Hrycyna CA,Young SG,Fong LG||Proceedings of the National Academy of Sciences of the United States of America (104:13432)||2007|
The farnesyltransferase inhibitor lonafarnib induces CCAAT/enhancer-binding protein homologous protein-dependent expression of death receptor 5, leading to induction of apoptosis in human cancer cells.
MA5-12745 was used in western blot to study the effect of farnesyltransferase inhibitor lonafarnib on death receptor 5 expression and apoptosis in human cancer cells
|Sun SY,Liu X,Zou W,Yue P,Marcus AI,Khuri FR||The Journal of biological chemistry (282:18800)||2007|
The farnesyltransferase inhibitor R115777 up-regulates the expression of death receptor 5 and enhances TRAIL-induced apoptosis in human lung cancer cells.
MA5-12745 was used in western blot to investigate the effect of farnesyltransferase inhibitor R115777 on death receptor 5 expression and apoptosis in human lung cancer cells
|Qiu Y,Liu X,Zou W,Yue P,Lonial S,Khuri FR,Sun SY||Cancer research (67:4973)||2007|
Heat shock protein 40/DjB1 is required for thermotolerance in early phase.
MA5-12745 was used in western blot to examine the role of heat shock protein 40/DjB1 in mammalian thermal response
|Uchiyama Y,Takeda N,Mori M,Terada K||Journal of biochemistry (140:805)||2006|
A phase I, pharmacokinetic, and biological study of the farnesyltransferase inhibitor tipifarnib in combination with gemcitabine in patients with advanced malignancies.
MA5-12745 was used in western blot to perform a phase I clinical trial of a farnesyltransferase inhibitor in combination with gemcitabine in patients with advanced malignancies
|Patnaik A,Eckhardt SG,Izbicka E,Tolcher AA,Hammond LA,Takimoto CH,Schwartz G,McCreery H,Goetz A,Mori M,Terada K,Gentner L,Rybak ME,Richards H,Zhang S,Rowinsky EK||Clinical cancer research : an official journal of the American Association for Cancer Research (9:4761)||2003|
A phase I and pharmacological study of the farnesyl protein transferase inhibitor L-778,123 in patients with solid malignancies.
MA5-12745 was used in western blot to report on a Phase I clinical trial of a peptidomimetic farnesyl protein transferase inhibitor in patients with solid malignancies
|Britten CD,Rowinsky EK,Soignet S,Patnaik A,Yao SL,Deutsch P,Lee Y,Lobell RB,Mazina KE,McCreery H,Pezzuli S,Spriggs D||Clinical cancer research : an official journal of the American Association for Cancer Research (7:3894)||2001|
Activity of the farnesyl protein transferase inhibitor SCH66336 against BCR/ABL-induced murine leukemia and primary cells from patients with chronic myeloid leukemia.
MA5-12745 was used in western blot to investigate the effect of farnesyl protein transferase inhibitor SCH66336 on BCR/ABL-induced murine leukemia and primary chronic myeloid leukemia cells
|Peters DG,Hoover RR,Gerlach MJ,Koh EY,Zhang H,Choe K,Kirschmeier P,Bishop WR,Daley GQ||Blood (97:1404)||2001|
Oculopharyngeal muscular dystrophy-like nuclear inclusions are present in normal magnocellular neurosecretory neurons of the hypothalamus.
MA5-12745 was used in immunocytochemistry to study oculopharyngeal muscular dystrophy-like nuclear inclusions in normal magnocellular neurosecretory neurons of the hypothalamus
|Berciano MT,Villagra NT,Ojeda JL,Navascues J,Gomes A,Lafarga M,Carmo-Fonseca M||Human molecular genetics (13:829)||2004|
developmentally down-regulated 7; DnaJ (Hsp40) homolog, subfamily A, member 1; dnaJ homolog subfamily A member 1; dnaJ protein homolog 2; DNAJ-like 2; dnaJ-like protein 1; DnaJ-like protein 2; DnaJ-like subfamily A member 1; heat shock 40 kDa protein 4; heat shock protein; heat shock protein J2; heat shock protein, DNAJ-like 2; HSJ-2; human DnaJ protein 2; neural precursor cell expressed; neural precursor cell expressed, developmentally down-regulated 7; neural precursor cell expressed, developmentally down-regulated gene 7
DJ-2; DjA1; DNAJ2; DNAJA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2; HSPF4; I79_019991; NEDD7; Rdj1