|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
|Western Blot (WB)||See 18 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Bacteria, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat|
|Published species reactivity||Rat , Non-human primate , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||N-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-12748 targets HDJ-2/DNAJ in FACS, IF, IHC (P), IP, and WB applications and shows reactivity with Bacteria, Hamster, Human, mouse, Non-human primate, Porcine, Rabbit, and Rat samples.
The MA5-12748 immunogen is n-terminal purified recombinant fragment (aa 1-179) of HDJ-2 fusion protein.
It is a novel human homolog of E. coli's DNAJ protein from heat shock protein 40 family. Hsp40 is involved in chaperonic protein folding HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane HDJ-2 has a prenylated C-terminus and is not inducible upon heat shock.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: developmentally down-regulated 7; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ-like 2; DnaJ-like protein 1; DnaJ-like protein 2; DnaJ-like subfamily A member 1; hDj-2; Heat shock 40 kDa protein 4; heat shock protein; Heat shock protein J2; heat shock protein, DNAJ-like 2; HSDJ; HSJ-2; Human DnaJ protein 2; neural precursor cell expressed; neural precursor cell expressed, developmentally down-regulated 7; neural precursor cell expressed, developmentally down-regulated gene 7
Gene Aliases: DJ-2; DjA1; DNAJ2; DNAJA1; hDJ-2; HDJ2; HSDJ; HSJ-2; HSJ2; HSPF4; I79_019991; NEDD7; Rdj1
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