Western blot analysis of HDL in human plasma protein using a HDL polyclonal antibody (Product # PA1-29508) at a dilution of 1:2000 followed by a Goat anti IgY HRP secondary antibody at a dilution of 1:1000. Colorimetric method was used for signal development. The lower MW band is Apolipoprotein AII and the higher MW band is Apolipoprotein AI.
|Tested species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Full length native plasma protein (purified) (Human).|
|Purification||Antigen affinity chromatography|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1500-1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29508 detects HDL from human samples.
PA1-29508 has been successfully used in Western blot applications.
The PA1-29508 immunogen is: Full length native plasma protein (purified) (Human).
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.