Immunofluorescent analysis of HEC1 in U2OS cells fixed by 4% PFA. Cells were costained using a HEC1 monoclonal antibody (Product # MA1-23308) at a dilution of 1:500 and an Alpha-Tubulin polyclonal antibody (Product # PA5-29444) (Green) at a dilution of 1:500. Chromosomes are stained with DAPI (blue). Scale bar, 5 um.
|Tested species reactivity||Hamster, Human, Mouse, Non-human primate, Pig|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Partial human HEC1 protein consisting of amino acids 56-642.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||4-10 ug/ml|
|Immunofluorescence (IF)||4-10 µg/ml|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||1-5 µg/ml|
|Western Blot (WB)||1:250-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-23308 detects HEC1 in human, mouse, pig, hamster and African green monkey samples.
MA1-23308 has been successfully used in immunofluorescence, immunoprecipitation, immunohistochemistry, flow cytometry and Western blot procedures. Positive control of HELA WCE suggested.
The MA1-23308 immunogen is partial human HEC1 protein consisting of amino acids 56-642.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The quantitative architecture of centromeric chromatin.
MA1-23308 was used in immunocytochemistry to study the localization of CENP-A in the cell and at centromeres.
|Bodor DL,Mata JF,Sergeev M,David AF,Salimian KJ,Panchenko T,Cleveland DW,Black BE,Shah JV,Jansen LE||eLife (3:null)||2014|