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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||GST-HEF-1 (82-398 aa.) from SP2/0 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Western Blot (WB)||1:2000-1:6000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA1-5784 detects HEF1 in human, mouse, and rat samples. This antibody does not recognize p130cas. It has not been tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites.
MA1-5784 has been successfully used in Western blot, immunoprecipitation, immunohistichemistry, and immunofluorescence procedures. For IHC, it is recommended to use human kidney as a positive control.
The MA1-5784 immunogen is GST-HEF-1(82-398aa.).
Store at 4°C for one month or at -20°C for longer periods.
HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
p75NTR-dependent Rac1 activation requires receptor cleavage and activation of an NRAGE and NEDD9 signaling cascade.
MA1-5784 was used in western blot to identify signaling cascades regulated by the p75 neurotrophin receptor.
|Zeinieh M,Salehi A,Rajkumar V,Barker PA||Journal of cell science (128:447)||2015|
A novel inhibitor of focal adhesion signaling induces caspase-independent cell death in diffuse large B-cell lymphoma.
MA1-5784 was used in western blot to investigate the role of a novel focal adhesion inhibitor of focal adhesion in the cell death of large B-cell lymphoma
|Bosch R,Dieguez-Gonzalez R,Céspedes MV,Parreño M,Pavón MÁ,Grañena A,Sierra J,Mangues R,Casanova I||Blood (118:4411)||2011|
Cas scaffolding protein family member 2; CAS-L; cas-like docking; CASL; Crk-associated substrate lymphocyte-specific protein; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ761I2.1; enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF1; neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated gene 9; p130Cas-related protein; renal carcinoma antigen NY-REN-12
CAS-L; CAS2; CASL; CASS2; HEF1; MEF1; Nedd-9; NEDD9; p105