|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||GST-HEF-1 (82-398 aa.) from SP2/0 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
CasL in human, mouse and rat samples. MA1-5785 does not recognize p130Cas. This antibody mostly detects HEF1 localized at the focal adhesion sites.
MA1-5785 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, and immunofluorescence procedures. Immunofluorescence was performed on 4% PFA fixed cells.
The MA1-5785 immunogen is GST-HEF-1(82-398aa.).
Store at 4°C for up to one month, and -20°C for long term storage.
HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A novel inhibitor of focal adhesion signaling induces caspase-independent cell death in diffuse large B-cell lymphoma.
MA1-5785 was used in western blot to investigate the role of a novel focal adhesion inhibitor of focal adhesion in the cell death of large B-cell lymphoma
|Bosch R,Dieguez-Gonzalez R,Céspedes MV,Parreño M,Pavón MÁ,Grañena A,Sierra J,Mangues R,Casanova I||Blood (118:4411)||2011|