Immunofluorescence analysis of HER-2 / ErbB2 was done on 70% confluent log phase T47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HER-2 / ErbB2 (L87 + 2ERB19) Mouse Monoclonal Antibody (MA511976) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cytoplasmic and membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Clone||L87 + 2ERB19|
|Immunogen||Extracellular domain of recombinant human c-erbB-2 oncoprotein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11976 targets HER-2 in WB applications and shows reactivity with Human samples.
The MA5-11976 immunogen is extracellular domain of recombinant human c-erbB-2 oncoprotein.
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ganoderma tsugae Extract Inhibits Growth of HER2-Overexpressing Cancer Cells via Modulation of HER2/PI3K/Akt Signaling Pathway.
MA5-11976 was used in immunohistochemistry, immunoprecipitation, and western blot to study the role of the HER2-PI3 kinase-Akt pathway in the inhibitory effects of a Gandoderma tsugae extract on the growth of cancer cells overexpressing HER2
|Kuo HP,Hsu SC,Ou CC,Li JW,Tseng HH,Chuang TC,Liu JY,Chen SJ,Su MH,Cheng YC,Chou WY,Kao MC||Evidence-based complementary and alternative medicine : eCAM (2013:null)||2013|
Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer.
MA5-11976 was used in immunohistochemistry to investigate the immune response elicited by the skin delivery of naked DNA vaccine
|Lin CC,Yen MC,Lin CM,Huang SS,Yang HJ,Chow NH,Lai MD||The journal of gene medicine (10:679)||2008|
Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer.
MA5-11976 was used in western blot to study the molecular mechanisms underlying the therapeutic actions of trastuzumab and pertuzumab against ovarian cancer
|Sims AH,Zweemer AJ,Nagumo Y,Faratian D,Muir M,Dodds M,Um I,Kay C,Hasmann M,Harrison DJ,Langdon SP||British journal of cancer (106:1779)||2012|
Shed HER2 extracellular domain in HER2-mediated tumor growth and in trastuzumab susceptibility.
MA5-11976 was used in western blot to study the role of the shed HER2 extracellular domain in HER2-mediated tumor growth and in trastuzumab susceptibility
|Ghedini GC,Ciravolo V,Tortoreto M,Giuffrè S,Bianchi F,Campiglio M,Mortarino M,Figini M,Coliva A,Carcangiu ML,Zambetti M,Piazza T,Ferrini S,Ménard S,Tagliabue E,Pupa SM||Journal of cellular physiology (225:256)||2010|
Expression of estrogen receptor alpha with a Tet-off adenoviral system induces G0/G1 cell cycle arrest in SKBr3 breast cancer cells.
MA5-11976 was used in western blot to investigate the effect of estrogen receptor alpha on breast cancer proliferation and cell cycle progression
|Peng J,Jordan VC||International journal of oncology (36:451)||2010|
Taxol increases the amount and T cell activating ability of self-immune stimulatory multimolecular complexes found in ovarian cancer cells.
MA5-11976 was used in western blot to study the effect of taxol on tumor antigen-specific immunity in ovarian cancer cells
|Tsuda N,Chang DZ,Mine T,Efferson C,García-Sastre A,Wang X,Ferrone S,Ioannides CG||Cancer research (67:8378)||2007|
Curcumin-induced degradation of ErbB2: A role for the E3 ubiquitin ligase CHIP and the Michael reaction acceptor activity of curcumin.
MA5-11976 was used in western blot to study the molecular mechanism of curcumin-induced degradation of ErbB
|Jung Y,Xu W,Kim H,Ha N,Neckers L||Biochimica et biophysica acta (1773:383)||2007|
Biosynthesis of tumorigenic HER2 C-terminal fragments by alternative initiation of translation.
MA5-11976 was used in western blot to study the generation of tumorigenic HER2 C-terminal fragments by alternative initiation of translation
|Anido J,Scaltriti M,Bech Serra JJ,Santiago Josefat B,Todo FR,Baselga J,Arribas J||The EMBO journal (25:3234)||2006|
Identification of ADAM10 as a major source of HER2 ectodomain sheddase activity in HER2 overexpressing breast cancer cells.
MA5-11976 was used in western blot to study the major role of ADAM10 in HER2 ectodomain shedding in HER2 overexpressing breast cancer cells
|Liu PC,Liu X,Li Y,Covington M,Wynn R,Huber R,Hillman M,Yang G,Ellis D,Marando C,Katiyar K,Bradley J,Abremski K,Stow M,Rupar M,Zhuo J,Li YL,Lin Q,Burns D,Xu M,Zhang C,Qian DQ,He C,Sharief V,Weng L,Agrios C,Shi E,Metcalf B,Newton R,Friedman S,Yao W,Scherle P,Hollis G,Burn TC||Cancer biology and therapy (5:657)||2006|
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
MA5-11976 was used in western blot to develop and characterize specific antibodies targeting EGFR family
|Horak E,Heitner T,Robinson MK,Simmons HH,Garrison J,Russeva M,Furmanova P,Lou J,Zhou Y,Yuan QA,Weiner LM,Adams GP,Marks JD||Cancer biotherapy and radiopharmaceuticals (20:603)||2005|
Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.
MA5-11976 was used in western blot to study the role of surface charge and hydrophobicity in ErbB2 binding to the Hsp90 chaperone complex
|Xu W,Yuan X,Xiang Z,Mimnaugh E,Marcu M,Neckers L||Nature structural and molecular biology (12:120)||2005|
Decreased accessibility and lack of activation of ErbB2 in JIMT-1, a herceptin-resistant, MUC4-expressing breast cancer cell line.
MA5-11976 was used in western blot to study the status of ErbB2 in a herceptin-resistant and MUC4-expressing breast cancer cell line
|Nagy P,Friedländer E,Tanner M,Kapanen AI,Carraway KL,Isola J,Jovin TM||Cancer research (65:473)||2005|
Alpha6beta1 integrin induces proteasome-mediated cleavage of erbB2 in breast cancer cells.
MA5-11976 was used in western blot to study the role of alpha6beta1 integrin in inducing proteasome-mediated cleavage of erbB2 in breast cancer cells
|Shimizu H,Seiki T,Asada M,Yoshimatsu K,Koyama N||Oncogene (22:831)||2003|
Role of MHC II affinity and molecular mimicry in defining anti-HER-2/neu MAb-3 linear peptide epitope.
MA5-11976 was used in western blot to study whether it is possible to predict which peptides will evoke a humoral immune response using the human HER-2/neu breast cancer-associated antigen as a model
|Lucchese A,Stevanovic S,Sinha AA,Mittelman A,Kanduc D||Peptides (24:193)||2003|
|Non-human primate||Not Cited||
Chaperone-dependent E3 ubiquitin ligase CHIP mediates a degradative pathway for c-ErbB2/Neu.
MA5-11976 was used in western blot to investigate the influence of CHIP on c-ErbB2/Neu degradation
|Xu W,Marcu M,Yuan X,Mimnaugh E,Patterson C,Neckers L||Proceedings of the National Academy of Sciences of the United States of America (99:12847)||2002|
Monoclonal and polyclonal humoral immune response to EC HER-2/NEU peptides with low similarity to the host's proteome.
MA5-11976 was used in western blot to study the immune response to EC HER-2/NEU peptides not shared by the host's proteome
|Mittelman A,Lucchese A,Sinha AA,Kanduc D||International journal of cancer (98:741)||2002|
Differential sensitivities of trastuzumab (Herceptin)-resistant human breast cancer cells to phosphoinositide-3 kinase (PI-3K) and epidermal growth factor receptor (EGFR) kinase inhibitors.
MA5-11976 was used in immunoprecipitation to investigate the mechanism for the trastuzumab resistence in human breast cancer treatment
|Chan CT,Metz MZ,Kane SE||Breast cancer research and treatment (91:187)||2005|