|Tested species reactivity||Rat, Human, Mouse|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to amino acids 153-433 of Human ErbB2|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28123 detects ErbB2 from rat, mouse, human samples.
PA1-28123 has been successfully used in IF/ICC and Western blot applications. A suggested positive control for this product is human breast caner cell line.
The PA1-28123 immunogen is synthetic peptide - amino acids 153-433 of Human ErbB2.
Store at 4°C short term. For extended storage, store at -20°C, avoiding freeze/thaw cycles.
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Oncogenesis Developmental processes Gametogenesis Oogenesis Embryogenesis Determination of dorsal/ventral axis Cell cycle Cell cycle control Apoptosis Inhibition of apoptosis Cell proliferation and differentiation