|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human c-erbB-2/HER-2 protein. This Sequence is identical in rat neu protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2.5-5.0 µg/ml|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16305 targets HER-2 in IHC (P), IP, and WB applications and shows reactivity with mouse, Non-human primate, Rat, and Human samples.
The PA5-16305 immunogen is synthetic peptide derived from C-terminus of human c-erbB-2/HER-2 protein. This Sequence is identical in rat neu protein.
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
IP-MS enrichment of ERBB2 (LFQ intensity): ERBB2 was enriched 978-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and ERBB2 antibody (Part No. PA5-16305). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The use of mouse models of breast cancer and quantitative image analysis to evaluate hormone receptor antigenicity after microwave-assisted formalin fixation.
PA5-16305 was used in immunohistochemistry to study hormone receptor immunogenicity following the use of rapid microwave-assisted formalin fixation of samples from murine models of breast cancer
|Engelberg JA,Giberson RT,Young LJ,Hubbard NE,Cardiff RD||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (62:319)||2014|
The mucin MUC4 and its membrane partner ErbB2 regulate biological properties of human CAPAN-2 pancreatic cancer cells via different signalling pathways.
PA5-16305 was used in immunoprecipitation and western blot to study the distinct mechanisms by which MUC4 and its membrane partner ErbB2 regulate biological properties of human CAPAN-2 pancreatic cancer cells
|Jonckheere N,Skrypek N,Merlin J,Dessein AF,Dumont P,Leteurtre E,Harris A,Desseyn JL,Susini C,Frénois F,Van Seuningen I||PloS one (7:null)||2012|