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Leinco Technologies

HER-2 (Trastuzumab) Recombinant Human Monoclonal Antibody (4D5-8)

View all (297) Her-2 (ErbB2) antibodies

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Product Details

LT1500-1MG

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Human / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4D5-8

Immunogen

Human epidermoid carcinoma cells (A431) over-expressing EGFR.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Amount

1 mg

Purification

Protein A/G

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4°C short term, -80°C long term

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Specificity: This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. Clone 4D5-8 recognizes human erbB-2. This product is for research use only.

Endotoxin Level: ≤ 1.0 EU/mg as determined by the LAL method. Purity: ≥95% monomer by analytical SEC.

Product Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing: To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling: Functional grade biosimilar antibodies may be stored sterile as received at 2-8 C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80 C. Avoid Repeated Freeze Thaw Cycles.

This antibody has been tested in the following applications: Immunohistochemistry, Flow Cytometry, ELISA, CyTOF®.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: approximately 68 kDa secreted autoinhibitor glycoprotein; c-erb B2/neu protein; CD antigen CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; HER2 receptor; HER2/neu receptor; herstatin; human epidermal growth factor receptor 2; metas; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; phospho ErbB2; proto-oncogene c-ErbB-2; proto-oncogene Neu; tyrosine kinase-type cell surface receptor HER2; unnamed protein product; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

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Gene Aliases: c-ERB-2; c-ERB2; CD340; HER-2; HER-2/neu; HER2; MLN 19; MLN-19; NEU; NGL; p185(erbB2); TKR1; VSCN2

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UniProt ID: (Human) P04626

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Entrez Gene ID: (Human) 2064

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Function(s)
RNA polymerase I core binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity transmembrane signaling receptor activity receptor binding protein binding ATP binding coreceptor activity growth factor binding receptor tyrosine kinase binding identical protein binding ErbB-3 class receptor binding protein heterodimerization activity
Process(es)
protein phosphorylation signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway cell proliferation Schwann cell development peptidyl-tyrosine phosphorylation neuron differentiation positive regulation of cell growth regulation of microtubule-based process positive regulation of Rho protein signal transduction intracellular signal transduction ERBB signaling pathway ERBB2 signaling pathway ERBB2-ERBB3 signaling pathway ERBB2-EGFR signaling pathway ERBB2-ERBB4 signaling pathway wound healing regulation of cell proliferation negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade protein kinase B signaling positive regulation of translation regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase I promoter positive regulation of JNK cascade positive regulation of epithelial cell proliferation regulation of ERK1 and ERK2 cascade cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus semaphorin-plexin signaling pathway positive regulation of protein targeting to membrane neurotransmitter receptor localization to postsynaptic specialization membrane
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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