Immunofluorescence analysis of HER-3 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HER-3 (2B5) Mouse Monoclonal Antibody (MA513040) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300).Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant rat c-erbB-3/HER-3 oncoprotein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13040 targets HER-3 in IP and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-13040 immunogen is recombinant rat c-erbB-3/HER-3 oncoprotein.
c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
IP-MS enrichment of ERBB3 (LFQ intensity): ERBB3 was enriched 231-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and ERBB3 antibody (Part No. MA5-13040). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oligomerization of the Nrdp1 E3 ubiquitin ligase is necessary for efficient autoubiquitination but not ErbB3 ubiquitination.
MA5-13040 was used in immunoprecipitation and western blot to study the requirement of Nrdp1 E3 ubiquitin ligase oligomer formation for self-ubiquitinylation by not for the unbiquitinylation of erbB3
|Printsev I,Yen L,Sweeney C,Carraway KL||The Journal of biological chemistry (289:8570)||2014|
The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2.
MA5-13040 was used in western blot to investigate the effect of the constitutive activity of EGFRvIII on wild-type EGFR and erbB2 in OVCA 433 cells
|Zeineldin R,Ning Y,Hudson LG||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (58:529)||2010|
Activation of nonreceptor tyrosine kinase Bmx/Etk mediated by phosphoinositide 3-kinase, epidermal growth factor receptor, and ErbB3 in prostate cancer cells.
MA5-13040 was used in western blot to study the mechanism of Bmx/Etk activation in prostate cancer cells
|Jiang X,Borgesi RA,McKnight NC,Kaur R,Carpenter CL,Balk SP||The Journal of biological chemistry (282:32689)||2007|
The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells.
MA5-13040 was used in western blot to investigate the role of ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells
|Liu W,Zscheppang K,Murray S,Nielsen HC,Dammann CE||Biochimica et biophysica acta (1772:737)||2007|
ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells.
MA5-13040 was used in western blot to study erbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells
|Zscheppang K,Korenbaum E,Bueter W,Ramadurai SM,Nielsen HC,Dammann CE||Pediatric pulmonology (41:1205)||2006|
HER3, p95HER2, and HER2 protein expression levels define multiple subtypes of HER2-positive metastatic breast cancer.
MA5-13040 was used in immunohistochemistry to identify multiple subtypes of HER2-positive metastatic breast cancer based on the quantitative expression analysis of HER3, p95HER2 and HER2 protein levels
|Lipton A,Goodman L,Leitzel K,Cook J,Sperinde J,Haddad M,Köstler WJ,Huang W,Weidler JM,Ali S,Newton A,Fuchs EM,Paquet A,Singer CF,Horvat R,Jin X,Banerjee J,Mukherjee A,Tan Y,Shi Y,Chenna A,Larson J,Lie Y,Sherwood T,Petropoulos CJ,Williams S,Winslow J,Parry G,Bates M||Breast cancer research and treatment (141:43)||2013|