Immunofluorescence analysis of HER-3 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ErbB3 (H3.105.5 (Ab105)) Mouse Monoclonal Antibody (MA513008) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Extracellular domain of recombinant human c-erbB-3/HER-3 oncoprotein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1:1-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13008 targets HER-3 in WB, flow cytometry, ICC/IF, and IP applications and shows reactivity with Human and mouse samples.
The MA5-13008 immunogen is extracellular domain of recombinant human c-erbB-3/HER-3 oncoprotein.
c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Synergistic Chemoimmunotherapy of Epithelial Ovarian Cancer Using ErbB-Retargeted T Cells Combined with Carboplatin.
MA5-13008 was used in flow cytometry to study the therapeutic potential in epithelial ovarian cancer of treatment with ErbB-retargeted T-cells plus carboplatin
|Parente-Pereira AC,Whilding LM,Brewig N,van der Stegen SJ,Davies DM,Wilkie S,van Schalkwyk MC,Ghaem-Maghami S,Maher J||Journal of immunology (Baltimore, Md. : 1950) (191:2437)||2013|
Flexible targeting of ErbB dimers that drive tumorigenesis by using genetically engineered T cells.
MA5-13008 was used in flow cytometry to develop a novel immunotherapeutic approach utilizing genetically engineered T-cells that target tumorigenic ErbB dimers
|Davies DM,Foster J,Van Der Stegen SJ,Parente-Pereira AC,Chiapero-Stanke L,Delinassios GJ,Burbridge SE,Kao V,Liu Z,Bosshard-Carter L,Van Schalkwyk MC,Box C,Eccles SA,Mather SJ,Wilkie S,Maher J||Molecular medicine (Cambridge, Mass.) (18:565)||2012|
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.
MA5-13008 was used in flow cytometry to investigate the status of the endothelial cell growth, cell death and fibrosis in aggressive inflammatory breast cancer
|Shirakawa K,Tsuda H,Heike Y,Kato K,Asada R,Inomata M,Sasaki H,Kasumi F,Yoshimoto M,Iwanaga T,Konishi F,Terada M,Wakasugi H||Cancer research (61:445)||2001|
Epidermal growth factor-like ligands differentially up-regulate matrix metalloproteinase 9 in head and neck squamous carcinoma cells.
MA5-13008 was used in flow cytometry to investigate the regulation of matrix metalloproteinase 9 by epidermal growth factor-like ligands in head and neck squamous carcinoma cells
|O-charoenrat P,Modjtahedi H,Rhys-Evans P,Court WJ,Box GM,Eccles SA||Cancer research (60:1121)||2000|
A Notch1-neuregulin1 autocrine signaling loop contributes to melanoma growth.
MA5-13008 was used in blocking or activating experiment to study the role of a Notch1-neuregulin1 autocrine signaling loop in melanoma growth
|Zhang K,Wong P,Zhang L,Jacobs B,Borden EC,Aster JC,Bedogni B||Oncogene (31:4609)||2012|
Decorin, erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 regulation of semaphorin 3A in central nervous system scar tissue.
MA5-13008 was used in blocking or activating experiment to study the role of decorin in suppressing semaphorin 3A expression within adult rat cerebral cortex scar tissue
|Minor KH,Bournat JC,Toscano N,Giger RJ,Davies SJ||Brain : a journal of neurology (134:1140)||2011|
The EGF/CSF-1 paracrine invasion loop can be triggered by heregulin beta1 and CXCL12.
MA5-13008 was used in blocking or activating experiment to study tumor invasion induced by heregulin beta1 and CXCL12 and the role of the EGF/CSF-1 paracrine loop
|Hernandez L,Smirnova T,Kedrin D,Wyckoff J,Zhu L,Stanley ER,Cox D,Muller WJ,Pollard JW,Van Rooijen N,Segall JE||Cancer research (69:3221)||2009|
Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons.
MA5-13008 was used in blocking or activating experiment to investigate the influence of ErbB localization on its signaling in hippocampal neurons
|Chen J,Tseng HC,Dichter MA,Zhang H,Greene MI||DNA and cell biology (24:553)||2005|
Signaling pathways required for matrix metalloproteinase-9 induction by betacellulin in head-and-neck squamous carcinoma cells.
MA5-13008 was used in blocking or activating experiment to investigate the mechanism of MMP9 induction by betacellulin in head-and-neck squamous carcinoma cells
|O-charoenrat P,Wongkajornsilp A,Rhys-Evans PH,Eccles SA||International journal of cancer (111:174)||2004|
Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth.
MA5-13008 was used in immunocytochemistry to investigate the role of Stat3 in the tumorigenic effect of heregulin/ErbB-2 in breast cells
|Proietti CJ,Rosemblit C,Beguelin W,Rivas MA,Díaz Flaqué MC,Charreau EH,Schillaci R,Elizalde PV||Molecular and cellular biology (29:1249)||2009|
ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells.
MA5-13008 was used in immunocytochemistry and western blot to study the role of ErbB4 in regulating fetal surfactant phospholipid synthesis in primary fetal rat type II cells
|Zscheppang K,Liu W,Volpe MV,Nielsen HC,Dammann CE||American journal of physiology. Lung cellular and molecular physiology (293:L429)||2007|
ErbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells.
MA5-13008 was used in immunocytochemistry to study erbB receptor dimerization, localization, and co-localization in mouse lung type II epithelial cells
|Zscheppang K,Korenbaum E,Bueter W,Ramadurai SM,Nielsen HC,Dammann CE||Pediatric pulmonology (41:1205)||2006|
Neuregulin expression, function, and signaling in human ovarian cancer cells.
MA5-13008 was used in immunocytochemistry to investigate the characteristics of neuregulin in human ovarian cancer cells
|Gilmour LM,Macleod KG,McCaig A,Sewell JM,Gullick WJ,Smyth JF,Langdon SP||Clinical cancer research : an official journal of the American Association for Cancer Research (8:3933)||2002|
The role of HER1-HER4 and EGFRvIII in hormone-refractory prostate cancer.
MA5-13008 was used in immunohistochemistry to study the role of HER1-HER4 and EGFRvIII in hormone-refractory prostate cancer
|Edwards J,Traynor P,Munro AF,Pirret CF,Dunne B,Bartlett JM||Clinical cancer research : an official journal of the American Association for Cancer Research (12:123)||2006|