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Western blot analysis of HER-4 was performed by loading 25 ug of mouse brain cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a HER-4 monoclonal antibody (Product # MA5-12888) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~180kDa.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12885 targets HER-4 in WB, FACS and IP applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-12885 immunogen is extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein.
c-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4).
MA5-12885 was used in immunohistochemistry to study the utility of the 4B5 monoclonal antibody for clinical HER2 status determination in breast tissue expressing HER4
|Jay JI,Brunhoeber PS,Smith MH,Williams RR,Sugarman MC,Free HL,Tast DE||Histopathology (62:563)||2013|
Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum.
MA5-12885 was used in immunohistochemistry to study the expression and immunolocalization of HB-EGF-like growth factor and EGFR in human corpus luteum
|Akayama Y,Takekida S,Ohara N,Tateiwa H,Chen W,Nakabayashi K,Maruo T||Human reproduction (Oxford, England) (20:2708)||2005|
Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.
MA5-12885 was used in immunocytochemistry to study the role of neuregulin in modulating ErbB4 endocytosis in cultured hippocampal neurons
|Longart M,Chatani-Hinze M,Gonzalez CM,Vullhorst D,Buonanno A||Brain research bulletin (73:210)||2007|
ErbB-4 ribozymes abolish neuregulin-induced mitogenesis.
MA5-12885 was used in immunocytochemistry to investigate the effect of ErbB-4 ribozymes on neuregulin-induced mitogenesis
|Tang CK,Goldstein DJ,Payne J,Czubayko F,Alimandi M,Wang LM,Pierce JH,Lippman ME||Cancer research (58:3415)||1998|
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
MA5-12885 was used in ELISA to develop and characterize specific antibodies targeting EGFR family
|Horak E,Heitner T,Robinson MK,Simmons HH,Garrison J,Russeva M,Furmanova P,Lou J,Zhou Y,Yuan QA,Weiner LM,Adams GP,Marks JD||Cancer biotherapy & radiopharmaceuticals (20:603)||2005|
HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization.
MA5-12885 was used in flow cytometry to study the role of HER-2/neu overexpression in increasing the viable hypoxic cell population within solid tumors
|Dragowska WH,Warburton C,Yapp DT,Minchinton AI,Hu Y,Waterhouse DN,Gelmon K,Skov K,Woo J,Masin D,Huxham LA,Kyle AH,Bally MB||Molecular cancer research : MCR (2:606)||2004|
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.
MA5-12885 was used in flow cytometry to investigate the status of the endothelial cell growth, cell death and fibrosis in aggressive inflammatory breast cancer
|Shirakawa K,Tsuda H,Heike Y,Kato K,Asada R,Inomata M,Sasaki H,Kasumi F,Yoshimoto M,Iwanaga T,Konishi F,Terada M,Wakasugi H||Cancer research (61:445)||2001|
Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol.
MA5-12885 was used in flow cytometry to study the role of the HER-2 receptor in modulating the response of ovarian cancer cells to doxorubicin, cisplatin and taxol
|Aigner A,Hsieh SS,Malerczyk C,Czubayko F||Toxicology (144:221)||2000|
Anti-HER2 antibody enhances the growth inhibitory effect of anti-oestrogen on breast cancer cells expressing both oestrogen receptors and HER2.
MA5-12885 was used in flow cytometry to investigate the synergistic inhibitory effect of anti-HER2 antibody and anti-oestrogen on breast cancer cells
|Kunisue H,Kurebayashi J,Otsuki T,Tang CK,Kurosumi M,Yamamoto S,Tanaka K,Doihara H,Shimizu N,Sonoo H||British journal of cancer (82:46)||2000|
avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4; Tyro-2