Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence analysis of HER-4 was done on 70% confluent log phase T47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HER-4 (H4.77.16 (Ab77)) Mouse Monoclonal Antibody (MA512888) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12888 targets HER-4 in Western blot, flow cytometry and immunoprecipitation applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-12888 immunogen is extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein.
c-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunohistochemical analysis of the monoclonal antibody 4B5 in breast tissue expressing human epidermal growth factor receptor 4 (HER4).
MA5-12888 was used in immunohistochemistry to study the utility of the 4B5 monoclonal antibody for clinical HER2 status determination in breast tissue expressing HER4
|Jay JI,Brunhoeber PS,Smith MH,Williams RR,Sugarman MC,Free HL,Tast DE||Histopathology (62:563)||2013|
Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum.
MA5-12888 was used in immunohistochemistry to study the expression and immunolocalization of HB-EGF-like growth factor and EGFR in human corpus luteum
|Akayama Y,Takekida S,Ohara N,Tateiwa H,Chen W,Nakabayashi K,Maruo T||Human reproduction (Oxford, England) (20:2708)||2005|
Regulation of ErbB-4 endocytosis by neuregulin in GABAergic hippocampal interneurons.
MA5-12888 was used in immunocytochemistry to study the role of neuregulin in modulating ErbB4 endocytosis in cultured hippocampal neurons
|Longart M,Chatani-Hinze M,Gonzalez CM,Vullhorst D,Buonanno A||Brain research bulletin (73:210)||2007|
Isolation of scFvs to in vitro produced extracellular domains of EGFR family members.
MA5-12888 was used in ELISA to develop and characterize specific antibodies targeting EGFR family
|Horak E,Heitner T,Robinson MK,Simmons HH,Garrison J,Russeva M,Furmanova P,Lou J,Zhou Y,Yuan QA,Weiner LM,Adams GP,Marks JD||Cancer biotherapy & radiopharmaceuticals (20:603)||2005|
HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization.
MA5-12888 was used in flow cytometry to study the role of HER-2/neu overexpression in increasing the viable hypoxic cell population within solid tumors
|Dragowska WH,Warburton C,Yapp DT,Minchinton AI,Hu Y,Waterhouse DN,Gelmon K,Skov K,Woo J,Masin D,Huxham LA,Kyle AH,Bally MB||Molecular cancer research : MCR (2:606)||2004|
Absence of endothelial cells, central necrosis, and fibrosis are associated with aggressive inflammatory breast cancer.
MA5-12888 was used in flow cytometry to investigate the status of the endothelial cell growth, cell death and fibrosis in aggressive inflammatory breast cancer
|Shirakawa K,Tsuda H,Heike Y,Kato K,Asada R,Inomata M,Sasaki H,Kasumi F,Yoshimoto M,Iwanaga T,Konishi F,Terada M,Wakasugi H||Cancer research (61:445)||2001|
Reversal of HER-2 over-expression renders human ovarian cancer cells highly resistant to taxol.
MA5-12888 was used in flow cytometry to study the role of the HER-2 receptor in modulating the response of ovarian cancer cells to doxorubicin, cisplatin and taxol
|Aigner A,Hsieh SS,Malerczyk C,Czubayko F||Toxicology (144:221)||2000|
Anti-HER2 antibody enhances the growth inhibitory effect of anti-oestrogen on breast cancer cells expressing both oestrogen receptors and HER2.
MA5-12888 was used in flow cytometry to investigate the synergistic inhibitory effect of anti-HER2 antibody and anti-oestrogen on breast cancer cells
|Kunisue H,Kurebayashi J,Otsuki T,Tang CK,Kurosumi M,Yamamoto S,Tanaka K,Doihara H,Shimizu N,Sonoo H||British journal of cancer (82:46)||2000|
avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; avian erythroblastosis oncogene B 4; c-erbB-4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; proto-oncogene-like protein c-ErbB-4; receptor tyrosine kinase; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4; Tyro-2