|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion Protein of HERG encoding 181 amino acid residues from the C-terminus.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-860 detects HERG in human samples.
PA3-860 has been used successfully in Western blot, immunfluorescence, and immunoprecipitation procedures. By Western blot this antibody detects the ~135 kDa and ~155 kDa forms of HERG.
The PA3-860 immunogen corresponds to a cDNA fragment of HERG encoding 181 amino acid residues from the C-terminus.
The human ether-a-go-go-related gene (HERG) encodes a voltage-gated potassium channel which has an important role in cardiac action potential repolarization in the mammalian heart. Mutations in HERG have been shown to cause chromosome 7-linked congenital long QT syndrome, a disorder associated with delayed cardiac repolarization, prolonged electrocardiographic QT intervals, and the development of ventricular arrhythmias. HERG channels are an important target for many drugs, and have emerged as a significant type of cardiac ion channel.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Chronic inhibition of cardiac Kir2.1 and HERG potassium channels by celastrol with dual effects on both ion conductivity and protein trafficking.
PA3-860 was used in western blot to study the effect of celastrol on cardiac Kir2.1 and hERG potassium channels.
|Sun H,Liu X,Xiong Q,Shikano S,Li M||The Journal of biological chemistry (281:5877)||2006|