Description: This 4H1HES monoclonal antibody reacts with human Hes1, a 30-kDa basic-helix-loop-helix transcriptional repressor. Hes1 is expressed in thymocytes, as well as neural, retinal, liver, and pancreatic tissues. Expression of Hes1 is controlled by Notch signaling, and thus plays an important role in T cell development. This regulation by Notch is also critical for neural stem cell differentiation and neurogenesis. Reports have demonstrated that Hes1 antagonizes transcription of MASH1, a basic-helix-loop-helix factor involved in neuronal determination. Finally, Hes1 has been associated with Notch-induced T cell acute lymphoblastic lymphoma and other cancers.
Applications Reported: This 4H1HES antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P).
Applications Tested: This 4H1HES antibody has been tested by immunohistochemistry on FFPE human kidney cortex at less than or equal to 20 µg/mL both without antigen retrieval or with high pH antigen retrieval. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHb39; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; HES-1; HHL; HRY; Transcription factor HES-1
Gene Aliases: BHLHB39; HES-1; HES1; HHL; HL; HRY
UniProt ID: (Human) Q14469
Entrez Gene ID: (Human) 3280