OriGene

HES1 Monoclonal Antibody (OTI4H1), TrueMAB™

View all (40) HES1 antibodies

Questions & Answers

Questions & Answers

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HES1 Antibody in Immunocytochemistry (ICC/IF) — Anti-HES1 mouse monoclonal antibody (TA500114) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HES1(RC211709).

HES1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of HeLa cells using anti-HES1 mouse monoclonal antibody (TA500114).

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-HES1 (TA000114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HES1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-HES1 (TA500114) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

HES1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1(RC211709) (RC211709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with an. Positive lysates LY417251 (100 µg) and LC417251 (20 µg) can be purchased separately from OriGene.

Product Details

TA500114

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI4H1
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1 View antigen
Conjugate	Unconjugated Biotin DyLight 488 HRP
Form	liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHb39; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; Hairy homolog; Hairy-like; Hairy-like protein; hHL; Transcription factor HES-1

Gene Aliases: BHLHB39; HES-1; HES1; HHL; HL; HRY

UniProt ID: (Human) Q14469

Entrez Gene ID: (Human) 3280

Molecular Function: basic helix-loop-helix transcription factor

negative regulation of transcription from RNA polymerase II promoter liver development embryonic heart tube morphogenesis outflow tract morphogenesis regulation of secondary heart field cardioblast proliferation ventricular septum development transcription, DNA-templated protein complex assembly cell adhesion Notch signaling pathway smoothened signaling pathway STAT protein import into nucleus nervous system development positive regulation of cell proliferation cell migration telencephalon development midbrain-hindbrain boundary morphogenesis oculomotor nerve development trochlear nerve development hindbrain morphogenesis forebrain radial glial cell differentiation adenohypophysis development lung development positive regulation of BMP signaling pathway midbrain development pancreas development somatic stem cell population maintenance ascending aorta morphogenesis positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of Stat3 protein auditory receptor cell fate determination positive regulation of DNA binding regulation of fat cell differentiation negative regulation of auditory receptor cell differentiation positive regulation of Notch signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of mitotic cell cycle, embryonic lateral inhibition positive regulation of JAK-STAT cascade cell maturation thymus development cell morphogenesis involved in neuron differentiation positive regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation artery morphogenesis regulation of epithelial cell proliferation inner ear receptor stereocilium organization regulation of timing of neuron differentiation negative regulation of glial cell proliferation ventricular septum morphogenesis ureteric bud morphogenesis labyrinthine layer blood vessel development common bile duct development negative regulation of stomach neuroendocrine cell differentiation cardiac neural crest cell development involved in outflow tract morphogenesis pharyngeal arch artery morphogenesis glomerulus vasculature development comma-shaped body morphogenesis S-shaped body morphogenesis renal interstitial fibroblast development metanephric nephron tubule morphogenesis cochlea development establishment of epithelial cell polarity vascular smooth muscle cell development neuronal stem cell population maintenance positive regulation of protein targeting to mitochondrion negative regulation of pancreatic A cell differentiation negative regulation of stem cell differentiation negative regulation of pro-B cell differentiation negative regulation of forebrain neuron differentiation

Process(es)

transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding chaperone binding N-box binding