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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23240 detects HET / SAF - B in Human and Mouse samples.
MA1-23240 has been successfully used in Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot procedures. Positive control of HeLa, MCF7 cells suggested.
MA1-23240 immunogen corresponds to A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein..
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DKFZp779C1727; glutathione S-transferase fusion protein; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein; HET; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF-B; SAF-B1; SAFB1; Scaffold attachment factor B1
3110021E02Rik; 5330423C17Rik; AU018122; D18386; E130307D12; HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB; SAFB1