|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human hepatocyte growth factor, expressed in the insect Sf 21 cells.|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||0.01-0.50 ug/ml|
|Neutralization (Neu)||0.01-0.5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24767 detects HGF from human samples.
MA1-24767 has been successfully used in neutralization, inhibition assays, ELISA, and Western blot procedures. Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF.
The MA1-24767 immunogen is recombinant human hepatocyte growth factor, expressed in the insect Sf 21 cells.
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.