|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, mouse hepatocyte growth factor receptor (HGFR).|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Immunohistochemistry (IHC)||Assay dependent|
|Neutralization (Neu)||0.3-1 µg/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20184 detects Hepatocyte Growth Factor Receptor in mouse samples and has been successfully used in ELISA, IHC, Neutralizing, and Western blot procedures.
The PA1-20184 immunogen is recombinant, mouse hepatocyte growth factor receptor (HGFR).
Hepatocyte growth factor receptor (HGF R), a product of the proto-oncogene c-Met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. The c-Met heterodimer is composed of an alpha chain that is disulfide-linked to a beta chain. Each alpha and beta subunit heterodimer contain 1152 amino acid residues with a calculated molecular mass of approximately 129 kDa. The alpha chain is exposed to the cell surface and the beta chain spans the plasma membrane. c-Met is synthesized as a single-chain precursor which undergoes cotranslational glycosylation and proteolytic cleavage producing the heterodimeric mature form. Human and mouse HGF receptors share 89% amino acid identity. HGF is the ligand for the HGF receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.