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HHV-1 TK Antibody (PA5-67984) in WB
Viruses 2021 -
Verification of acyclovir-resistant (ACV R ) HSV-1 infection in Vero cells. ( A ) Flow chart of the methodology used to create ACV-resistant (ACV R ) mutants, adapted from Sarisky et al. []. Verification of acyclovir-resistant (ACV R )-GFP-HSV-1 17+ in Vero cells. ( B ) Fluorescent images of 0.1 MOI GFP-HSV-1 infection in Vero cells with or without 45 uM ACV. FITC filter with excitation of 490 nm and emissi... View More
Product Details
PA5-67984
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from human KITH_HHV11.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2691521
Target Information
The Thymidine kinase catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. The thymidine kinase allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors. It also phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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