Invitrogen

HIBCH Monoclonal Antibody (OTI3H5)

Product Image Gallery

HIBCH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a HIBCH monoclonal antibody (Product # MA5-25150) at a dilution of 1:50.

HIBCH Antibody in Western Blot (WB) — Western blot analysis of HIBCH in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with HIBCH (Product # MA5-25150) monoclonal antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Western Blot (WB)

1:1000-1:2000

Product Specifications

Species Reactivity

Dog, Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI3H5

Immunogen

Full length human recombinant protein of HIBCH produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724444

Target Information

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3-hydroxyisobutyryl-CoA hydrolase; 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial; 3-hydroxyisobutyryl-Coenzyme A hydrolase; HIB-CoA hydrolase; HIBYL-CoA-H; HIBYLCOAH; mitochondrial; testicular tissue protein Li 86

Gene Aliases: HIBCH; HIBYLCOAH

UniProt ID: (Human) Q6NVY1

Entrez Gene ID: (Dog) 607040, (Human) 26275

Molecular Function: acetyltransferase acyltransferase dehydrogenase epimerase/racemase hydratase isomerase ligase lyase oxidoreductase transferase