Invitrogen

HIBCH Monoclonal Antibody (OTI3H5)

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HIBCH Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a HIBCH monoclonal antibody (Product # MA5-25150) at a dilution of 1:50.

HIBCH Antibody in Western Blot (WB) — Western blot analysis of HIBCH in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with HIBCH (Product # MA5-25150) monoclonal antibody.

Product Details

MA5-25150

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Dog, Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI3H5
Immunogen	Full length human recombinant protein of HIBCH produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.9 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724444

Target Information

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial; 3-hydroxyisobutyryl-Coenzyme A hydrolase; HIB-CoA hydrolase; HIBYL-CoA-H; testicular tissue protein Li 86

Gene Aliases: HIBCH; HIBYLCOAH

UniProt ID: (Human) Q6NVY1

Entrez Gene ID: (Dog) 607040, (Human) 26275

Molecular Function: hydrolase