OriGene

HIBCH Monoclonal Antibody (OTI5C5), TrueMAB™

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HIBCH Antibody in Immunocytochemistry (ICC/IF) — Anti-HIBCH mouse monoclonal antibody (TA501361) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH(RC209814).

HIBCH Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH. Positive lysates LY402324 (100 µg) and LC402324 (20 µg) can be purchased separately from OriGene.

HIBCH Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC209814 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HIBCH antibody (TA501361), and then analyzed by flow cytometry.

Product Details

TA501361

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Dog, Human, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5C5
Immunogen	Full length human recombinant protein of human HIBCH produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.79 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial; 3-hydroxyisobutyryl-Coenzyme A hydrolase; HIB-CoA hydrolase; HIBYL-CoA-H; testicular tissue protein Li 86

Gene Aliases: HIBCH; HIBYLCOAH

UniProt ID: (Human) Q6NVY1, (Rat) Q5XIE6

Entrez Gene ID: (Dog) 607040, (Human) 26275, (Rat) 301384

Molecular Function: hydrolase