|Tested species reactivity||Bovine, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast|
|Published species reactivity||Rabbit, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Fusion protein containing residues 432-528 of human HIF-1alpha.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Gel Shift (GS)||1:1-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.
For WB, testing on nuclear extracts is recommended.
Suggested positive control: Cos7 CoCl2-treated nuclear extract.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells.
MA1-16504 was used in western blot to characterize the association with RASAL1 promoter hypermethylation in human coronary endothelial cells and hypoxia-induced endothelial-mesenchymal transition
|Xu X,Tan X,Hulshoff MS,Wilhelmi T,Zeisberg M,Zeisberg EM||FEBS letters (590:1222)||2016|
Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism.
MA1-16504 was used in western blot to study the mechanisms underlying the beneficial effects of a chromone glycoside on the repair of burn wounds
|Sumiyoshi M,Kimura Y||Phytomedicine : international journal of phytotherapy and phytopharmacology (17:820)||2010|
Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma.
MA1-16504 was used in western blot to study changes in vascular parameters, growth factors and tumor size following treatment of rat glioma with a VEGFR tyrosine kinase inhibitor
|Ali MM,Janic B,Babajani-Feremi A,Varma NR,Iskander AS,Anagli J,Arbab AS||PloS one (5:null)||2010|
Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model.
MA1-16504 was used in western blot to study the mechanisms underlying the antitumor effects of 2-oxoglutarate in a murine tumor model
|Matsumoto K,Obara N,Ema M,Horie M,Naka A,Takahashi S,Imagawa S||Cancer science (100:1639)||2009|
DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells.
MA1-16504 was used in western blot to study the role of DNA-dependent protein kinase in the accumulation of hypoxia-inducible factor-1alpha in HepG2 cells
|Kang MJ,Jung SM,Kim MJ,Bae JH,Kim HB,Kim JY,Park SJ,Song HS,Kim DW,Kang CD,Kim SH||The FEBS journal (275:5969)||2008|
Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway.
MA1-16504 was used in western blot to study the mechanism by which Akt1 activation can augment HIF-1alpha expression
|Pore N,Jiang Z,Shu HK,Bernhard E,Kao GD,Maity A||Molecular cancer research : MCR (4:471)||2006|
HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart.
MA1-16504 was used in western blot to study the involvement of hypoxia-inducible factor-1alpha-targeted pathways in heat acclimation
|Maloyan A,Eli-Berchoer L,Semenza GL,Gerstenblith G,Stern MD,Horowitz M||Physiological genomics (23:79)||2005|
Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.
MA1-16504 was used in western blot to study the effects of hypoxia and HIF-1alpha antisense oligonucleotides on cancer cells
|Wang Y,Pakunlu RI,Tsao W,Pozharov V,Minko T||Molecular pharmaceutics (1:156)||2005|
HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin.
MA1-16504 was used in western blot to investigate the function of HLF in a mouse model of retinopathy of prematurity
|Morita M,Ohneda O,Yamashita T,Takahashi S,Suzuki N,Nakajima O,Kawauchi S,Ema M,Shibahara S,Udono T,Tomita K,Tamai M,Sogawa K,Yamamoto M,Fujii-Kuriyama Y||The EMBO journal (22:1134)||2003|
Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.
MA1-16504 was used in western blot to study the enhanced expression of the novel protein Cap24 in human cancer cells and its potential diagnostic value
|Cangul H,Salnikow K,Yee H,Zagzag D,Commes T,Costa M||Cell biology and toxicology (18:87)||2002|
Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.
MA1-16504 was used in western blot to study the role of histone deacetylases in the regulation of hypoxia-induced angiogenesis
|Kim MS,Kwon HJ,Lee YM,Baek JH,Jang JE,Lee SW,Moon EJ,Kim HS,Lee SK,Chung HY,Kim CW,Kim KW||Nature medicine (7:437)||2001|
Hyperinducibility of hypoxia-responsive genes without p53/p21-dependent checkpoint in aggressive prostate cancer.
MA1-16504 was used in western blot to investigate the role of the hypoxia-responsive genes in tumor growth and metastasis
|Salnikow K,Costa M,Figg WD,Blagosklonny MV||Cancer research (60:5630)||2000|
Prognostic impact of clinicopathological features and expression of biomarkers related to (18)F-FDG uptake in esophageal cancer.
MA1-16504 was used in immunohistochemistry - paraffin section to correlate 18-F-fluoro-deoxyglucose uptake and aggressive tumor biology in predicting outcome in esophageal cancer
|Schreurs LM,Smit JK,Pavlov K,Pultrum BB,Pruim J,Groen H,Hollema H,Plukker JT||Annals of surgical oncology (21:3751)||2014|
The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
MA1-16504 was used in immunohistochemistry to study the expression and role of CXCR2 and SOCS3 in renal cell carcinoma aggresiveness and metastasis
|Stofas A,Levidou G,Piperi C,Adamopoulos C,Dalagiorgou G,Bamias A,Karadimou A,Lainakis GA,Papadoukakis S,Stravodimos K,Dimopoulos MA,Patsouris E,Gakiopoulou H,Korkolopoulou P||BMC cancer (14:null)||2014|
The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma.
MA1-16504 was used in immunohistochemistry to study the value of analysing VHL/HIF pathway components for predicting the therapeutic response of metastatic clear-cell renal cell carconima patients to pazopanib
|Choueiri TK,Fay AP,Gagnon R,Lin Y,Bahamon B,Brown V,Rosenberg JE,Hutson TE,Baker-Neblett KL,Carpenter C,Liu Y,Pandite L,Signoretti S||Clinical cancer research : an official journal of the American Association for Cancer Research (19:5218)||2013|
Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging.
MA1-16504 was used in immunohistochemistry to study the immunohistochemical expression of a range of biomarkers in order to select a panel suitable for targeted intra-operative imaging of servere endometriosis
|van den Berg LL,Crane LM,van Oosten M,van Dam GM,Simons AH,Hofker HS,Bart J||International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics (121:35)||2013|
Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1¿ (HIF-1¿).
MA1-16504 was used in immunohistochemistry to study the role of low-level laser therapy in neuropathic pain alleviation and recovery from chronic constriction injury
|Hsieh YL,Chou LW,Chang PL,Yang CC,Kao MJ,Hong CZ||The Journal of comparative neurology (520:2903)||2012|
KAI1 suppresses HIF-1¿ and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer.
MA1-16504 was used in immunohistochemistry to study the mechanism by which the KAI1 metastasis-suppressor gene reduces expression of HIF-1alpha and VEGF in prostate cancer
|Park JJ,Jin YB,Lee YJ,Lee JS,Lee YS,Ko YG,Lee M||BMC cancer (12:null)||2012|
Immunohistochemical study of the angiogenetic network of VEGF, HIF1¿, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
MA1-16504 was used in immunohistochemistry to study the expression of VEGF, VEGFR-2, HIF1-alpha and eNOS in invasive ductal carcinoma
|Kafousi M,Vrekoussis T,Tsentelierou E,Pavlakis K,Navrozoglou I,Dousias V,Sanidas E,Tsiftsis D,Georgoulias V,Stathopoulos EN||Pathology oncology research : POR (18:33)||2012|
Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice.
MA1-16504 was used in immunohistochemistry to study the effect of cigarette smoke on noise-induced hearing loss and subsequent recovery in a mouse model
|Ahn JH,Joo HS,Suh JK,Kim H,So HS,Chung JW||Otology and neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology (32:926)||2011|
Expression of thioredoxin-1 and hypoxia inducible factor-1¿ in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property.
MA1-16504 was used in immunohistochemistry to study the expression and roles of thioredoxin-1 and HIF-1alpha in cerebral arteriovenous malformations
|Takagi Y,Kikuta K,Moriwaki T,Aoki T,Nozaki K,Hashimoto N,Miyamoto S||Surgical neurology international (2:null)||2011|
HIF-1¿ and VEGF are associated with disease progression in esophageal carcinoma.
MA1-16504 was used in immunohistochemistry to investigate the relationship between specific gene expression and esophageal cancer progression
|Takala H,Saarnio J,Wiik H,Ohtonen P,Soini Y||The Journal of surgical research (167:41)||2011|
Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha.
MA1-16504 was used in immunohistochemistry to study the role of N-Myc and hypoxia inducible factor HIF-1alpha in the regulation of neuroblastoma tumor progression
|Qing G,Skuli N,Mayes PA,Pawel B,Martinez D,Maris JM,Simon MC||Cancer research (70:10351)||2010|
Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors.
MA1-16504 was used in immunohistochemistry to investigate the distribution of receptor tyrosine kinase KIT in endothelial cells of young brain tumors
|Puputti M,Tynninen O,Pernilä P,Salmi M,Jalkanen S,Paetau A,Sihto H,Joensuu H||Brain pathology (Zurich, Switzerland) (20:763)||2010|
Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma.
MA1-16504 was used in immunohistochemistry to demonstrate the prognostic significance of vasculogenic mimicry in gastric adenocarcinoma
|Li M,Gu Y,Zhang Z,Zhang S,Zhang D,Saleem AF,Zhao X,Sun B||Pathology oncology research : POR (16:259)||2010|
Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.
MA1-16504 was used in immunohistochemistry to study the relationship between HIF-1alpha expression and various prognostic parameters in bladder urothelial carcinomas
|Deniz H,Karakök M,Yagci F,Güldür ME||International urology and nephrology (42:103)||2010|
Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.
MA1-16504 was used in immunohistochemistry to study the effects of ethanol and tobacco smoke on hepatic steatosis and hypoxia in hypercholesterolemic apoE(-/-) mice
|Bailey SM,Mantena SK,Millender-Swain T,Cakir Y,Jhala NC,Chhieng D,Pinkerton KE,Ballinger SW||Free radical biology and medicine (46:928)||2009|
Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis.
MA1-16504 was used in immunohistochemistry to study epithelial cell turnover and HIF expression in acute acalculous and calculous cholecystitis
|Vakkala M,Laurila JJ,Saarnio J,Koivukangas V,Syrjälä H,Karttunen T,Soini Y,Ala-Kokko TI||Critical care (London, England) (11:null)||2008|
Endothelial cell KIT expression in human tumours.
MA1-16504 was used in immunohistochemistry to study the expression of endothelial cell KIT in a wide of human tumors
|Sihto H,Tynninen O,Bützow R,Saarialho-Kere U,Joensuu H||The Journal of pathology (211:481)||2007|
Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion.
MA1-16504 was used in immunohistochemistry to examine the role of hypoxia-inducible factor 1 and VEGF in the development and progression of glioblastoma
|Zagzag D,Lukyanov Y,Lan L,Ali MA,Esencay M,Mendez O,Yee H,Voura EB,Newcomb EW||Laboratory investigation; a journal of technical methods and pathology (86:1221)||2006|
Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system.
MA1-16504 was used in immunohistochemistry to study the role of the HIF-1alpha/VEGF system in the enhanced bone formation caused by axial shortening during distraction osteogenesis in a rabbit model
|Mori S,Akagi M,Kikuyama A,Yasuda Y,Hamanishi C||Journal of orthopaedic research : official publication of the Orthopaedic Research Society (24:653)||2006|
Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.
MA1-16504 was used in immunohistochemistry to study the expression of hypoxia-inducible factor 1alpha and VEGF in hepatocellular carcinoma
|Huang GW,Yang LY,Lu WQ||World journal of gastroenterology (11:1705)||2005|
Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
MA1-16504 was used in immunohistochemistry to study the effect of noise stimulation on HIF-1alpha in mouse inner ear
|Chung JW,Kang HH,Shin JE,Kim JU||Neuroreport (15:2353)||2004|
HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array.
MA1-16504 was used in immunohistochemistry to study the gene expression patterns in human skeletal muscle obtained from lower limbs amputated due ischemia
|Tuomisto TT,Rissanen TT,Vajanto I,Korkeela A,Rutanen J,Ylä-Herttuala S||Atherosclerosis (174:111)||2004|
Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene.
MA1-16504 was used in immunohistochemistry to study the effects of conditional inactivation of the VHL gene in mice
|Ma W,Tessarollo L,Hong SB,Baba M,Southon E,Back TC,Spence S,Lobe CG,Sharma N,Maher GW,Pack S,Vortmeyer AO,Guo C,Zbar B,Schmidt LS||Cancer research (63:5320)||2003|
Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium.
MA1-16504 was used in immunohistochemistry to investigate the therapeutic efficacy of prostaglandin E1 in ischemic heart disease
|Mehrabi MR,Serbecic N,Tamaddon F,Pacher R,Horvath R,Mall G,Glogar HD||Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie (57:173)||2003|
Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease.
MA1-16504 was used in immunohistochemistry to investigate the influence of PGE-1 on myocardic angiogenesis in patients with ischemic heart disease
|Mehrabi MR,Serbecic N,Tamaddon F,Kaun C,Huber K,Pacher R,Wild T,Mall G,Wojta J,Glogar HD||Cardiovascular research (56:214)||2002|
Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.
MA1-16504 was used in immunohistochemistry to study the role of HIF-1/hypoxia-related protein Cap43 overexpression in cancer cells
|Cangul H,Salnikow K,Yee H,Zagzag D,Commes T,Costa M||Environmental health perspectives (110 Suppl 5:783)||2002|
Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk.
MA1-16504 was used in immunocytochemistry to study the role of HIF-1alpha in glycolytic metabolism and the expression of aggrecan in nucleus pulposus cells of the rat intervertebral disc
|Agrawal A,Guttapalli A,Narayan S,Albert TJ,Shapiro IM,Risbud MV||American journal of physiology. Cell physiology (293:C621)||2007|
Understanding hypoxia-induced gene expression in early development: in vitro and in vivo analysis of hypoxia-inducible factor 1-regulated zebra fish insulin-like growth factor binding protein 1 gene expression.
MA1-16504 was used in EMSA to investigate the hypoxia-induced gene expression in early development
|Kajimura S,Aida K,Duan C||Molecular and cellular biology (26:1142)||2006|
Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes.
MA1-16504 was used in EMSA to investigate the existence of hypoxia response elements in the promoter of DEC1 and DEC2
|Miyazaki K,Kawamoto T,Tanimoto K,Nishiyama M,Honda H,Kato Y||The Journal of biological chemistry (277:47014)||2002|
Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP.
MA1-16504 was used in EMSA to study the role of HIF-1, AP-1, GATA-2, and p300/CBP in the regulation of the endothelin-1 gene by hypoxia
|Yamashita K,Discher DJ,Hu J,Bishopric NH,Webster KA||The Journal of biological chemistry (276:12645)||2001|