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On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
|Tested species reactivity||Avian, Bovine, Dog, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rat|
|Published species reactivity||Rabbit, Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Fusion protein containing amino acids 432-528 of human HIF-1alpha|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:300|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.
For WB, testing on nuclear extracts is recommended.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
MA1-46457 was used in immunohistochemistry to study the expression and role of CXCR2 and SOCS3 in renal cell carcinoma aggresiveness and metastasis
|Stofas A,Levidou G,Piperi C,Adamopoulos C,Dalagiorgou G,Bamias A,Karadimou A,Lainakis GA,Papadoukakis S,Stravodimos K,Dimopoulos MA,Patsouris E,Gakiopoulou H,Korkolopoulou P||BMC cancer (14:null)||2014|
The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma.
MA1-46457 was used in immunohistochemistry to study the value of analysing VHL/HIF pathway components for predicting the therapeutic response of metastatic clear-cell renal cell carconima patients to pazopanib
|Choueiri TK,Fay AP,Gagnon R,Lin Y,Bahamon B,Brown V,Rosenberg JE,Hutson TE,Baker-Neblett KL,Carpenter C,Liu Y,Pandite L,Signoretti S||Clinical cancer research : an official journal of the American Association for Cancer Research (19:5218)||2013|
Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging.
MA1-46457 was used in immunohistochemistry to study the immunohistochemical expression of a range of biomarkers in order to select a panel suitable for targeted intra-operative imaging of servere endometriosis
|van den Berg LL,Crane LM,van Oosten M,van Dam GM,Simons AH,Hofker HS,Bart J||International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics (121:35)||2013|
KAI1 suppresses HIF-1α and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer.
MA1-46457 was used in immunohistochemistry to study the mechanism by which the KAI1 metastasis-suppressor gene reduces expression of HIF-1alpha and VEGF in prostate cancer
|Park JJ,Jin YB,Lee YJ,Lee JS,Lee YS,Ko YG,Lee M||BMC cancer (12:null)||2012|
Immunohistochemical study of the angiogenetic network of VEGF, HIF1α, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
MA1-46457 was used in immunohistochemistry to study the expression of VEGF, VEGFR-2, HIF1-alpha and eNOS in invasive ductal carcinoma
|Kafousi M,Vrekoussis T,Tsentelierou E,Pavlakis K,Navrozoglou I,Dousias V,Sanidas E,Tsiftsis D,Georgoulias V,Stathopoulos EN||Pathology oncology research : POR (18:33)||2012|
Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice.
MA1-46457 was used in immunohistochemistry to study the effect of cigarette smoke on noise-induced hearing loss and subsequent recovery in a mouse model
|Ahn JH,Joo HS,Suh JK,Kim H,So HS,Chung JW||Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology (32:926)||2011|
Expression of thioredoxin-1 and hypoxia inducible factor-1α in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property.
MA1-46457 was used in immunohistochemistry to study the expression and roles of thioredoxin-1 and HIF-1alpha in cerebral arteriovenous malformations
|Takagi Y,Kikuta K,Moriwaki T,Aoki T,Nozaki K,Hashimoto N,Miyamoto S||Surgical neurology international (2:null)||2011|
Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha.
MA1-46457 was used in immunohistochemistry to study the role of N-Myc and hypoxia inducible factor HIF-1alpha in the regulation of neuroblastoma tumor progression
|Qing G,Skuli N,Mayes PA,Pawel B,Martinez D,Maris JM,Simon MC||Cancer research (70:10351)||2010|
Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors.
MA1-46457 was used in immunohistochemistry to investigate the distribution of receptor tyrosine kinase KIT in endothelial cells of young brain tumors
|Puputti M,Tynninen O,Pernilä P,Salmi M,Jalkanen S,Paetau A,Sihto H,Joensuu H||Brain pathology (Zurich, Switzerland) (20:763)||2010|
Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma.
MA1-46457 was used in immunohistochemistry to demonstrate the prognostic significance of vasculogenic mimicry in gastric adenocarcinoma
|Li M,Gu Y,Zhang Z,Zhang S,Zhang D,Saleem AF,Zhao X,Sun B||Pathology oncology research : POR (16:259)||2010|
Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.
MA1-46457 was used in immunohistochemistry to study the relationship between HIF-1alpha expression and various prognostic parameters in bladder urothelial carcinomas
|Deniz H,Karakök M,Yagci F,Güldür ME||International urology and nephrology (42:103)||2010|
Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.
MA1-46457 was used in immunohistochemistry to study the effects of ethanol and tobacco smoke on hepatic steatosis and hypoxia in hypercholesterolemic apoE(-/-) mice
|Bailey SM,Mantena SK,Millender-Swain T,Cakir Y,Jhala NC,Chhieng D,Pinkerton KE,Ballinger SW||Free radical biology & medicine (46:928)||2009|
Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis.
MA1-46457 was used in immunohistochemistry to study epithelial cell turnover and HIF expression in acute acalculous and calculous cholecystitis
|Vakkala M,Laurila JJ,Saarnio J,Koivukangas V,Syrjälä H,Karttunen T,Soini Y,Ala-Kokko TI||Critical care (London, England) (11:null)||2008|
Endothelial cell KIT expression in human tumours.
MA1-46457 was used in immunohistochemistry to study the expression of endothelial cell KIT in a wide of human tumors
|Sihto H,Tynninen O,Bützow R,Saarialho-Kere U,Joensuu H||The Journal of pathology (211:481)||2007|
Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion.
MA1-46457 was used in immunohistochemistry to examine the role of hypoxia-inducible factor 1 and VEGF in the development and progression of glioblastoma
|Zagzag D,Lukyanov Y,Lan L,Ali MA,Esencay M,Mendez O,Yee H,Voura EB,Newcomb EW||Laboratory investigation; a journal of technical methods and pathology (86:1221)||2006|
Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system.
MA1-46457 was used in immunohistochemistry to study the role of the HIF-1alpha/VEGF system in the enhanced bone formation caused by axial shortening during distraction osteogenesis in a rabbit model
|Mori S,Akagi M,Kikuyama A,Yasuda Y,Hamanishi C||Journal of orthopaedic research : official publication of the Orthopaedic Research Society (24:653)||2006|
Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.
MA1-46457 was used in immunohistochemistry to study the expression of hypoxia-inducible factor 1alpha and VEGF in hepatocellular carcinoma
|Huang GW,Yang LY,Lu WQ||World journal of gastroenterology (11:1705)||2005|
Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
MA1-46457 was used in immunohistochemistry to study the effect of noise stimulation on HIF-1alpha in mouse inner ear
|Chung JW,Kang HH,Shin JE,Kim JU||Neuroreport (15:2353)||2004|
HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array.
MA1-46457 was used in immunohistochemistry to study the gene expression patterns in human skeletal muscle obtained from lower limbs amputated due ischemia
|Tuomisto TT,Rissanen TT,Vajanto I,Korkeela A,Rutanen J,Ylä-Herttuala S||Atherosclerosis (174:111)||2004|
Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene.
MA1-46457 was used in immunohistochemistry to study the effects of conditional inactivation of the VHL gene in mice
|Ma W,Tessarollo L,Hong SB,Baba M,Southon E,Back TC,Spence S,Lobe CG,Sharma N,Maher GW,Pack S,Vortmeyer AO,Guo C,Zbar B,Schmidt LS||Cancer research (63:5320)||2003|
Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.
MA1-46457 was used in immunohistochemistry to study the role of HIF-1/hypoxia-related protein Cap43 overexpression in cancer cells
|Cangul H,Salnikow K,Yee H,Zagzag D,Commes T,Costa M||Environmental health perspectives (110 Suppl 5:783)||2002|
Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism.
MA1-46457 was used in western blot to study the mechanisms underlying the beneficial effects of a chromone glycoside on the repair of burn wounds
|Sumiyoshi M,Kimura Y||Phytomedicine : international journal of phytotherapy and phytopharmacology (17:820)||2010|
Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma.
MA1-46457 was used in western blot to study changes in vascular parameters, growth factors and tumor size following treatment of rat glioma with a VEGFR tyrosine kinase inhibitor
|Ali MM,Janic B,Babajani-Feremi A,Varma NR,Iskander AS,Anagli J,Arbab AS||PloS one (5:null)||2010|
Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model.
MA1-46457 was used in western blot to study the mechanisms underlying the antitumor effects of 2-oxoglutarate in a murine tumor model
|Matsumoto K,Obara N,Ema M,Horie M,Naka A,Takahashi S,Imagawa S||Cancer science (100:1639)||2009|
Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway.
MA1-46457 was used in western blot to study the mechanism by which Akt1 activation can augment HIF-1alpha expression
|Pore N,Jiang Z,Shu HK,Bernhard E,Kao GD,Maity A||Molecular cancer research : MCR (4:471)||2006|
Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.
MA1-46457 was used in western blot to study the effects of hypoxia and HIF-1alpha antisense oligonucleotides on cancer cells
|Wang Y,Pakunlu RI,Tsao W,Pozharov V,Minko T||Molecular pharmaceutics (1:156)||2005|
HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin.
MA1-46457 was used in western blot to investigate the function of HLF in a mouse model of retinopathy of prematurity
|Morita M,Ohneda O,Yamashita T,Takahashi S,Suzuki N,Nakajima O,Kawauchi S,Ema M,Shibahara S,Udono T,Tomita K,Tamai M,Sogawa K,Yamamoto M,Fujii-Kuriyama Y||The EMBO journal (22:1134)||2003|
Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.
MA1-46457 was used in western blot to study the enhanced expression of the novel protein Cap24 in human cancer cells and its potential diagnostic value
|Cangul H,Salnikow K,Yee H,Zagzag D,Commes T,Costa M||Cell biology and toxicology (18:87)||2002|
Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.
MA1-46457 was used in western blot to study the role of histone deacetylases in the regulation of hypoxia-induced angiogenesis
|Kim MS,Kwon HJ,Lee YM,Baek JH,Jang JE,Lee SW,Moon EJ,Kim HS,Lee SK,Chung HY,Kim CW,Kim KW||Nature medicine (7:437)||2001|
Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk.
MA1-46457 was used in immunocytochemistry to study the role of HIF-1alpha in glycolytic metabolism and the expression of aggrecan in nucleus pulposus cells of the rat intervertebral disc
|Agrawal A,Guttapalli A,Narayan S,Albert TJ,Shapiro IM,Risbud MV||American journal of physiology. Cell physiology (293:C621)||2007|
ARNT intera; ARNT interacting protein; ARNT-interacting protein; basic-helix-loop-helix-PAS protein MOP1; class E basic helix-loop-helix protein 78; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; Hif1a; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8
AA959795; BHLHE78; BOS_11009; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8