|Tested species reactivity||Dog, Guinea pig, Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing amino acids 432-528 of human HIF-1alpha|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||25 µl/1.5x10^7 cells|
|Gel Shift (GS)||1:1-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
For ChIP, nuclear extracts are recommended.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: Cos-7 Hypoxia Induced nuclear extract Kit6.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mouse snail is a target gene for HIF.
PA3-16521 was used in immunoprecipitation to investigate the relationship between transcriptional inhibitor Snail and hypoxia-inducible factor in mice
|Luo D,Wang J,Li J,Post M||Molecular cancer research : MCR (9:234)||2011|