|Tested species reactivity||Bovine, Ferret, Human, Mouse, Non-human primate, Sheep, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Fusion protein containing residues 496-789 of human ARNT.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for ARNT (transient overexpression lysate).
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. (1,5,6)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart.
MA1-16512 was used in western blot to study the involvement of hypoxia-inducible factor-1alpha-targeted pathways in heat acclimation
|Maloyan A,Eli-Berchoer L,Semenza GL,Gerstenblith G,Stern MD,Horowitz M||Physiological genomics (23:79)||2005|
Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes.
MA1-16512 was used in EMSA to investigate the existence of hypoxia response elements in the promoter of DEC1 and DEC2
|Miyazaki K,Kawamoto T,Tanimoto K,Nishiyama M,Honda H,Kato Y||The Journal of biological chemistry (277:47014)||2002|