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Invitrogen
HIF-1 beta Monoclonal Antibody (2B10)
FIGURE: 1 / 9
HIF-1 beta Antibody (MA1-515) in ICC/IF
Immunofluorescent analysis of HIF-1 beta (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HIF-1 beta antibody (Product # MA1-515) at a dilution of 40 µg/mL in staining buffer for 1 hour at room temperatur... View More
Product Details
MA1-515
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Chimpanzee,
Fish,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2B10
Immunogen
Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2059441
Product Specific Information
MA1-515 detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT) from human, mouse, rat, and primate tissues.
MA1-515 has been successfully used in Western blot, immunofluorescence, gel shift, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an 87 kDa protein representing ARNT in COS cell extract. Immunofluorescence staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using MA1-515 yields non-nucleolar, nuclear staining only.
MA1-515 immunogen is a synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Target Information
HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARNT protein; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; hif 1; HIF-1 beta; HIF-1-beta; HIF1 beta; HIF1-beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta
Gene Aliases: ARNT; Arnt1; BHLHE2; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO; W08714
UniProt ID: (Human) Q5QP39, (Rat) P41739, (Mouse) P53762
Entrez Gene ID: (Human) 405, (Rat) 25242, (Mouse) 11863
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
aryl hydrocarbon receptor activity
protein binding
transcription factor binding
aryl hydrocarbon receptor binding
enhancer binding
sequence-specific DNA binding
protein heterodimerization activity
transcription factor activity, transcription factor binding
DNA binding
signal transducer activity
protein dimerization activity
basic helix-loop-helix transcription factor
response to hypoxia
embryonic placenta development
positive regulation of endothelial cell proliferation
transcription, DNA-templated
positive regulation of vascular endothelial growth factor production
cell differentiation
intracellular receptor signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of protein sumoylation
mRNA transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of erythrocyte differentiation
positive regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
response to toxic substance
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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