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Invitrogen
HIF-1 beta Monoclonal Antibody (2B10)
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FIGURE: 1 / 9
HIF-1 beta Antibody (MA1-515) in ICC/IF
Immunofluorescent analysis of HIF-1 beta (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HIF-1 beta antibody (Product # MA1-515) at a dilution of 40 µg/mL in staining buffer for 1 hour at room temperatur... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-515
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Chimpanzee,
Fish,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2B10
Immunogen
Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-515 detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT) from human, mouse, rat, and primate tissues.
MA1-515 has been successfully used in Western blot, immunofluorescence, gel shift, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an 87 kDa protein representing ARNT in COS cell extract. Immunofluorescence staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using MA1-515 yields non-nucleolar, nuclear staining only.
MA1-515 immunogen is a synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Target Information
HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aryl hydrocarbon receptor nuclear translocator 1; basic helix-loop-helix transcription factor; class E basic helix-loop-helix protein 2; dioxin receptor, nuclear translocator; hif 1; HIF-1-beta; HIF1 beta; HIF1-beta; hypoxia inducible factor 1 subunit beta; hypoxia-inducible factor 1, beta subunit; hypoxia-inducible factor 1-beta; unnamed protein product
Gene Aliases: ARNT1; bHLHe2; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO
UniProt ID: (Human) P27540, (Rat) P41739, (Mouse) P53762
Entrez Gene ID: (Human) 405, (Rat) 25242, (Mouse) 11863
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
aryl hydrocarbon receptor binding
protein homodimerization activity
sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
macromolecular complex binding
response to hypoxia
embryonic placenta development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
circadian rhythm
response to toxic substance
positive regulation of vascular endothelial growth factor production
cell differentiation
intracellular receptor signaling pathway
positive regulation of protein sumoylation
cellular response to oxidative stress
positive regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
nitric oxide metabolic process
positive regulation of hormone biosynthetic process
negative regulation of inflammatory response
G1 to G0 transition
response to dexamethasone
positive regulation of endothelial cell proliferation
xenobiotic metabolic process
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of erythrocyte differentiation
cellular response to hypoxia
intestinal epithelial structure maintenance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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