|ChIP assay (ChIP)||1-3 µl|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Virus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Tat protein of HIV-1 BH10 purified from E. coli|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody reacts with HIV-1 Tat BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1 subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). It has been mapped to amino acids 6-12 (PRLEPWK - B subtype a.a. consensus).
MA1-71509 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat.
HIV-1 Tat ("Trans-Activator of Transcription") consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: HIV-1 Tat; Human immunodeficiency virus
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.