|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant p15 protein|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4958 detects the HIV-1 p15 protein in HIV samples.
PA1-4958 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~15 kDa protein (HIV-1 p15) in HIV samples.
PA1-4958 immunogen corresponds to the recombinant p15 protein which was over-expressed in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus.
Recombinant HIV-1 Gag p15 protein (Cat. # RP-4918 or RP-4919) can be purchased for control experiments in Western blot assays.
HIV-1 Gag p15 is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by the binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.