|Tested species reactivity||Virus|
|Host / Isotype||E.coli|
|Storage buffer||TBS, pH 7.5, with 50% glycerol, 0.01M beta mercaptoethanol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RP-4917 can be used as a positive control in Western blotting with ABR antibody anti-HIV-1 p24 (PA1-4951, PA1-4952, or PA1-4953), as well as a p24 antigen in detection of anti-HIV-1 p24 antibody in Western blotting or ELISA. Also, it can be used as standard in the titration of p24 antigens as it indicates the AIDS virus amount in virus infection, diagnosis and treatment progress
RP-4917 has a calculated molecular weight of ~24 kDa. Purity is 90%.
HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element in the virus particle construction. As this protein is detectable from the early stage of AIDS virus infection, it is used as a marker for observation of the development in the patient and quote;s condition after treatment, as it indicates the amount of virus in the blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.