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|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant p15 protein|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4960 detects the HIV-1 p55 protein in HIV samples.
PA1-4960 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~55 kDa protein, corresponding to HIV-1 p55 in HIV samples. Also detected are a ~15 kDa, ~17 kDa and ~24 kDa protein, corresponding to the digested proteins.
PA1-4960 immunogen corresponds to the recombinant p55 protein which was over-expressed in E. coli with a plasmid carrying the Gag p55 coding region of HIV-1 virus.
Recombinant HIV-1 Gag p55 protein (Cat. # RP-4920 or RP-4921) can be purchased for control experiments in Western blot assays.
HIV-1 Gag p55 is a precursor protein of several proteins that form the core structure of AIDS virus, indispensable to its reproduction. P55 is digested by HIV-1 protease, first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1, both of unknown function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.