|Tested species reactivity||Bovine, Human, Non-human primate, Rodent|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified human tonsil lymphocyte membranes.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:50-1:100|
|Functional Assay (FN)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with amphibia, chicken, dog, goat, guinea pig, mouse or rabbit.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is tonsil. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis C virus infection in inclusion body myositis: A case-control study.
MA1-80123 was used in immunohistochemistry - paraffin section to determine if there is an association between inclusion body myositis and hepatitis C virus infection
|Uruha A,Noguchi S,Hayashi YK,Tsuburaya RS,Yonekawa T,Nonaka I,Nishino I||Neurology (86:211)||2016|
Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.
MA1-80123 was used in immunocytochemistry to study the role of clathrin-dependent and clathrin-independent routes in the uptake of Helicobacter pylori vesicles by gastric epithelial cells
|Olofsson A,Nygård Skalman L,Obi I,Lundmark R,Arnqvist A||mBio (5:e00979)||2014|
Alterations in the Arf6-regulated plasma membrane endosomal recycling pathway in cells overexpressing the tetraspan protein Gas3/PMP22.
MA1-80123 was used in immunocytochemistry to study alterations in Arf6-regulated plasma membrane endosomal recycling in cells overexpressing Gas3/PMP22
|Chies R,Nobbio L,Edomi P,Schenone A,Schneider C,Brancolini C||Journal of cell science (116:987)||2003|
Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.
MA1-80123 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells
|Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK||Anticancer research (33:4875)||2013|
Induction of metastatic cancer stem cells from the NK/LAK-resistant floating, but not adherent, subset of the UP-LN1 carcinoma cell line by IFN-¿.
MA1-80123 was used in flow cytometry to study the induction of metastatic cancer stem cells from UP-LN1 cell line
|Chen HC,Chou AS,Liu YC,Hsieh CH,Kang CC,Pang ST,Yeh CT,Liu HP,Liao SK||Laboratory investigation; a journal of technical methods and pathology (91:1502)||2011|
|Not Applicable||Not Cited||
The human herpesvirus-7 (HHV-7) U21 immunoevasin subverts NK-mediated cytoxicity through modulation of MICA and MICB.
MA1-80123 was used in western blot to report that the human herpesvirus-7 U21 gene product interferes with natural killer cell recognition
|Schneider CL,Hudson AW||PLoS pathogens (7:null)||2011|
Establishment of a quantitative ELISA capable of determining peptide - MHC class I interaction.
MA1-80123 was used in ELISA to develop a quantitative ELISA assay for studying peptide-MHC class I interactions
|Sylvester-Hvid C,Kristensen N,Blicher T,Ferré H,Lauemøller SL,Wolf XA,Lamberth K,Nissen MH,Pedersen LØ,Buus S||Tissue antigens (59:251)||2002|