Product References
NLRC5-CIITA Fusion Protein as an Effective Inducer of MHC-I Expression and Antitumor Immunity.
International journal of molecular sciences
Santharam MA,Shukla A,Levesque D,Kufer TA,Boisvert FM,Ramanathan S,Ilangumaran S
12-9983-42 was used in Flow Cytometry to propose that NLRC5-SA, with its ability to increase tumor immunogenicity and promote tumor growth control, could overcome the limitations of NLRC5-FL for translational immunotherapy applications.
Thu Apr 13 00:00:00 UTC 2023
Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistribution.
Molecular therapy. Methods & clinical development
Cabanes-Creus M,Navarro RG,Liao SHY,Scott S,Carlessi R,Roca-Pinilla R,Knight M,Baltazar G,Zhu E,Jones M,Denisenko E,Forrest ARR,Alexander IE,Tirnitz-Parker JEE,Lisowski L
12-9983-42 was used in Flow cytometry/Cell sorting to show that fine-tuning the HSPG attachment profile of AAV vectors can be a powerful tool for engineering their liver lobular transduction profiles.
Thu Mar 09 00:00:00 UTC 2023
AAV-p40 Bioengineering Platform for Variant Selection Based on Transgene Expression.
Human gene therapy
Westhaus A,Cabanes-Creus M,Jonker T,Sallard E,Navarro RG,Zhu E,Baltazar Torres G,Lee S,Wilmott P,Gonzalez-Cordero A,Santilli G,Thrasher AJ,Alexander IE,Lisowski L
12-9983-42 was used in flow cytometry to discover that AAV-p40 is a ubiquitously active promoter that can be modified for cell-type-specific expression by incorporating binding sites for silencing transcription factors, allowing for cell-type-specific library selection.
Wed Jun 01 00:00:00 UTC 2022
Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.
Nature communications
Piekarska K,Urban-Wójciuk Z,Kurkowiak M,Pelikant-Małecka I,Schumacher A,Sakowska J,Spodnik JH,Arcimowicz Ł,Zielińska H,Tymoniuk B,Renkielska A,Siebert J,Słomińska E,Trzonkowski P,Hupp T,Marek-Trzonkowska NM
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Mon Feb 14 00:00:00 UTC 2022
Novel human liver-tropic AAV variants define transferable domains that markedly enhance the human tropism of AAV7 and AAV8.
Molecular therapy. Methods & clinical development
Cabanes-Creus M,Navarro RG,Zhu E,Baltazar G,Liao SHY,Drouyer M,Amaya AK,Scott S,Nguyen LH,Westhaus A,Hebben M,Wilson LOW,Thrasher AJ,Alexander IE,Lisowski L
12-9983-42 was used in Flow Cytometry to underscore the potential of AAV-SYDs as liver gene therapy vectors and provide insights into the mechanism responsible for their enhanced transduction profile.
Thu Mar 10 00:00:00 UTC 2022
Screening HLA-A-restricted T cell epitopes of SARS-CoV-2 and the induction of CD8+ T cell responses in HLA-A transgenic mice.
Cellular & molecular immunology
Jin X,Ding Y,Sun S,Wang X,Zhou Z,Liu X,Li M,Chen X,Shen A,Wu Y,Liu B,Zhang J,Li J,Yang Y,Qiu H,Shen C,He Y,Zhao G
12-9983-42 was used in Flow Cytometry to functionally validate T cell epitopes and the development of vaccines that induce specific T cell responses.
Wed Dec 01 00:00:00 UTC 2021
Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.
Nucleic acids research
Li D,Sun X,Yu F,Perle MA,Araten D,Boeke JD
12-9983-42 was used in Flow Cytometry to serve as a general platform with multiple selection markers and may be particularly useful for large scale genome engineering projects such as Genome Project-Write (GP-write).
Thu Mar 18 00:00:00 UTC 2021
Frequent amplification of HDAC genes and efficacy of HDAC inhibitor chidamide and PD-1 blockade combination in soft tissue sarcoma.
Journal for immunotherapy of cancer
Que Y,Zhang XL,Liu ZX,Zhao JJ,Pan QZ,Wen XZ,Xiao W,Xu BS,Hong DC,Guo TH,Shen LJ,Fan WJ,Chen HY,Weng DS,Xu HR,Zhou PH,Zhang YZ,Niu XH,Zhang X
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Mon Feb 01 00:00:00 UTC 2021
The effects of T-DXd on the expression of HLA class I and chemokines CXCL9/10/11 in HER2-overexpressing gastric cancer cells.
Scientific reports
Nakajima S,Mimura K,Matsumoto T,Thar Min AK,Ito M,Nakano H,Neupane P,Kanke Y,Okayama H,Saito M,Momma T,Watanabe Y,Hanayama H,Hayase S,Saze Z,Kono K
12-9983-42 was used in Flow cytometry/Cell sorting to suggest that T-DXd triggers anti-tumor immune responses at least in part through induction of the expression of HLA class I and CXCL9/10/11 on HER2-positive GC cells, resulting in the enhancement of anti-tumor immunity in human gastric cancer (GC).
Thu Aug 19 00:00:00 UTC 2021
Restoring the natural tropism of AAV2 vectors for human liver.
Science translational medicine
Cabanes-Creus M,Hallwirth CV,Westhaus A,Ng BH,Liao SHY,Zhu E,Navarro RG,Baltazar G,Drouyer M,Scott S,Logan GJ,Santilli G,Bennett A,Ginn SL,McCaughan G,Thrasher AJ,Agbandje-McKenna M,Alexander IE,Lisowski L
12-9983-42 was used in Flow Cytometry to derive naturally hepatotropic AAV capsid sequences from primary human liver samples.
Wed Sep 09 00:00:00 UTC 2020
High-Throughput In Vitro, Ex Vivo, and In Vivo Screen of Adeno-Associated Virus Vectors Based on Physical and Functional Transduction.
Human gene therapy
Westhaus A,Cabanes-Creus M,Rybicki A,Baltazar G,Navarro RG,Zhu E,Drouyer M,Knight M,Albu RF,Ng BH,Kalajdzic P,Kwiatek M,Hsu K,Santilli G,Gold W,Kramer B,Gonzalez-Cordero A,Thrasher AJ,Alexander IE,Lisowski L
12-9983 was used in Flow cytometry/Cell sorting to investigate the transduction characteristics of adeno-associated virus vectors using high-throughput screening.
Fri May 01 00:00:00 UTC 2020
Attenuation of Heparan Sulfate Proteoglycan Binding Enhances In Vivo Transduction of Human Primary Hepatocytes with AAV2.
Molecular therapy. Methods & clinical development
Cabanes-Creus M,Westhaus A,Navarro RG,Baltazar G,Zhu E,Amaya AK,Liao SHY,Scott S,Sallard E,Dilworth KL,Rybicki A,Drouyer M,Hallwirth CV,Bennett A,Santilli G,Thrasher AJ,Agbandje-McKenna M,Alexander IE,Lisowski L
12-9983 was used in Flow cytometry/Cell sorting to investigate if enhanced heparan sulfate proteoglycan binding can negatively affect the in vivo function of otherwise strongly hepatotropic variants and if modulation of the interaction with heparan sulfate proteoglycan is critical to ensure maximum efficiency in vivo.
Fri Jun 12 00:00:00 UTC 2020
Attenuation of Heparan Sulfate Proteoglycan Binding Enhances In Vivo Transduction of Human Primary Hepatocytes with AAV2.
Molecular therapy. Methods & clinical development
Cabanes-Creus M,Westhaus A,Navarro RG,Baltazar G,Zhu E,Amaya AK,Liao SHY,Scott S,Sallard E,Dilworth KL,Rybicki A,Drouyer M,Hallwirth CV,Bennett A,Santilli G,Thrasher AJ,Agbandje-McKenna M,Alexander IE,Lisowski L
12-9983 was used in Flow cytometry/Cell sorting to investigate if enhanced heparan sulfate proteoglycan binding can negatively affect the in vivo function of otherwise strongly hepatotropic variants and if modulation of the interaction with heparan sulfate proteoglycan is critical to ensure maximum efficiency in vivo.
Fri Jun 12 00:00:00 UTC 2020
hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.
EBioMedicine
Todorova D,Zhang Y,Chen Q,Liu J,He J,Fu X,Xu Y
12-9983 was used in Flow cytometry/Cell sorting to investigate whether the adoptive transfer of CTLA4-Ig/PD-L1 knock-in dendritic cell-like cells(CP DCLs) can induce long-term immune tolerance of parental human embryonic stem cells(hESC)-derived smooth muscle and cardiomyocyte allografts.
Tue Dec 01 00:00:00 UTC 2020
PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.
Stem cell research & therapy
Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J
12-9983-42 was used in Flow cytometry/Cell sorting to investigate the role of Pluronic F-127 (PF-127) hydrogel plus antioxidant sodium ascorbyl phosphate (SAP) in enhancing Wharton's jelly mesenchymal stem cell (WJMSC)-mediated effectiveness on full-thickness skin wound healing in mice.
Fri Apr 03 00:00:00 UTC 2020
Combining DNMT and HDAC6 inhibitors increases anti-tumor immune signaling and decreases tumor burden in ovarian cancer.
Scientific reports
Moufarrij S,Srivastava A,Gomez S,Hadley M,Palmer E,Austin PT,Chisholm S,Diab N,Roche K,Yu A,Li J,Zhu W,Lopez-Acevedo M,Villagra A,Chiappinelli KB
12-9983-42 was used in Flow Cytometry to find that combining DNMTi and HDAC6i results in an amplified type I interferon response, leading to increased cytokine and chemokine expression and higher expression of the MHC I antigen presentation complex in human and mouse ovarian cancer cell lines.
Wed Feb 26 00:00:00 UTC 2020
Multiple Knockout of Classical HLA Class II β-Chains by CRISPR/Cas9 Genome Editing Driven by a Single Guide RNA.
Journal of immunology (Baltimore, Md. : 1950)
Crivello P,Ahci M,Maaßen F,Wossidlo N,Arrieta-Bolaños E,Heinold A,Lange V,Falkenburg JHF,Horn PA,Fleischhauer K,Heinrichs S
12-9983 was used in Flow cytometry/Cell sorting to investigate the effects of CRISPR/Cas9 genome editing to simultaneously target HLA-DRB, -DQB1, and -DPB1 through a single guide RNA.
Fri Mar 15 00:00:00 UTC 2019
Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.
JCI insight
Dawson NA,Lamarche C,Hoeppli RE,Bergqvist P,Fung VC,McIver E,Huang Q,Gillies J,Speck M,Orban PC,Bush JW,Mojibian M,Levings MK
12-9983 was used in Flow cytometry/Cell sorting to characterise and test alloantigen-specific chimeric antigen receptors in regulatory T cells.
Thu Mar 21 00:00:00 UTC 2019
Exploration of the Combination of PLK1 Inhibition with Immunotherapy in Cancer Treatment.
Journal of oncology
Li M,Liu Z,Wang X
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Thu Mar 31 00:00:00 UTC 2022
Exploration of the Combination of PLK1 Inhibition with Immunotherapy in Cancer Treatment.
Journal of oncology
Li M,Liu Z,Wang X
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Thu Mar 31 00:00:00 UTC 2022
AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.
Oncology letters
Xiu W,Ma J,Lei T,Zhang M
Sat Sep 01 00:00:00 UTC 2018
Hypoxia and hypoxia-inducible factor (HIF) downregulate antigen-presenting MHC class I molecules limiting tumor cell recognition by T cells.
PloS one
Sethumadhavan S,Silva M,Philbrook P,Nguyen T,Hatfield SM,Ohta A,Sitkovsky MV
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Wed Dec 06 00:00:00 UTC 2017
Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT.
Scientific reports
Shen C,Xu T,Wu Y,Li X,Xia L,Wang W,Shahzad KA,Zhang L,Wan X,Qiu J
12-9983 was used in Flow cytometry/Cell sorting to facilitate the routine analysis of patient-specific cellular immune response pattern to a given antigen in translational studies.
Mon Nov 27 00:00:00 UTC 2017
Immune profiling of NF1-associated tumors reveals histologic subtype distinctions and heterogeneity: implications for immunotherapy.
Oncotarget
Haworth KB,Arnold MA,Pierson CR,Choi K,Yeager ND,Ratner N,Roberts RD,Finlay JL,Cripe TP
12-9983 was used in Flow cytometry/Cell sorting to define the immunologic profiles of benign and malignant NF1-related tumours.
Tue Oct 10 00:00:00 UTC 2017
Inhibiting DNA methylation activates cancer testis antigens and expression of the antigen processing and presentation machinery in colon and ovarian cancer cells.
PloS one
Siebenkäs C,Chiappinelli KB,Guzzetta AA,Sharma A,Jeschke J,Vatapalli R,Baylin SB,Ahuja N
12-9983 was used in Flow cytometry/Cell sorting to demonstrate the role of epigenetics in dampening the presentation of cancer testis antigens in colon and ovarian cancer.
Thu Sep 14 00:00:00 UTC 2017
Identification and validation of multiple cell surface markers of clinical-grade adipose-derived mesenchymal stromal cells as novel release criteria for good manufacturing practice-compliant production.
Stem cell research & therapy
Camilleri ET,Gustafson MP,Dudakovic A,Riester SM,Garces CG,Paradise CR,Takai H,Karperien M,Cool S,Sampen HJ,Larson AN,Qu W,Smith J,Dietz AB,van Wijnen AJ
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Thu Aug 11 00:00:00 UTC 2016
Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types.
PloS one
Momose F,Seo N,Akahori Y,Sawada S,Harada N,Ogura T,Akiyoshi K,Shiku H
12-9983 was used in Flow cytometry/Cell sorting to statistically analyse miRNA microarray data, revealing that guanine-rich sequences are a dominant feature of exosome-dominant miRNAs.
Wed Apr 05 00:00:00 UTC 2017
KIR3DS1-Specific D0 Domain Polymorphisms Disrupt KIR3DL1 Surface Expression and HLA Binding.
Journal of immunology (Baltimore, Md. : 1950)
Mulrooney TJ,Zhang AC,Goldgur Y,Boudreau JE,Hsu KC
12-9983 was used in Flow cytometry/Cell sorting to analyse the functional impact of KIR3DS1-specific polymorphisms on KIR3DL1*009 surface expression, binding to HLA, and functional capacity.
Sat Aug 01 00:00:00 UTC 2015
Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.
Oncotarget
Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Tue Oct 06 00:00:00 UTC 2015
Inhibition of mitogen-activated protein kinase pathway can induce upregulation of human leukocyte antigen class I without PD-L1-upregulation in contrast to interferon-γ treatment.
Cancer science
Mimura K,Kua LF,Shiraishi K,Kee Siang L,Shabbir A,Komachi M,Suzuki Y,Nakano T,Yong WP,So J,Kono K
12-9983 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which HLA class I and PD-L1 are regulated by MAPK inhibitors and interferon-γ.
Wed Oct 01 00:00:00 UTC 2014
Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.
Stem cells (Dayton, Ohio)
Fernandez I,Ooi TP,Roy K
Published figure using HLA-ABC monoclonal antibody (Product # 12-9983-42) in Flow Cytometry
Wed Jan 01 00:00:00 UTC 2014
A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.
PloS one
Abramowski P,Ogrodowczyk C,Martin R,Pongs O
12-9983 was used in Flow cytometry/Cell sorting to show that P2RX5 gene expression of human T lymphocytes is upregulated during activation.
Mon May 18 00:00:00 UTC 2015
Age-associated cross-reactive antibody-dependent cellular cytotoxicity toward 2009 pandemic influenza A virus subtype H1N1.
The Journal of infectious diseases
Jegaskanda S,Laurie KL,Amarasena TH,Winnall WR,Kramski M,De Rose R,Barr IG,Brooks AG,Reading PC,Kent SJ
12-9983 was used in cell sorting to assess whether pre-existing antibodies with effector functions provided partial immunity to older individuals during the 2009 influenza A virus H1N1 pandemic.
Tue Oct 01 00:00:00 UTC 2013
Alterations of immune response of Non-Small Cell Lung Cancer with Azacytidine.
Oncotarget
Wrangle J,Wang W,Koch A,Easwaran H,Mohammad HP,Vendetti F,Vancriekinge W,Demeyer T,Du Z,Parsana P,Rodgers K,Yen RW,Zahnow CA,Taube JM,Brahmer JR,Tykodi SS,Easton K,Carvajal RD,Jones PA,Laird PW,Weisenberger DJ,Tsai S,Juergens RA,Topalian SL,Rudin CM,Brock MV,Pardoll D,Baylin SB
12-9983 was used in Flow cytometry/Cell sorting to define a biomarker strategy to examine if epigenetic therapy can sensitize patients with non-small cell lung cancer to PD-1 immune checkpoint blockade.
Fri Nov 01 00:00:00 UTC 2013
NK cells impede glioblastoma virotherapy through NKp30 and NKp46 natural cytotoxicity receptors.
Nature medicine
Alvarez-Breckenridge CA,Yu J,Price R,Wojton J,Pradarelli J,Mao H,Wei M,Wang Y,He S,Hardcastle J,Fernandez SA,Kaur B,Lawler SE,Vivier E,Mandelboim O,Moretta A,Caligiuri MA,Chiocca EA
12-9983 was used in Flow cytometry/Cell sorting to demonstrate that glioblastoma virotherapy is limited partially by an antiviral NK cell response involving specific NCRs, uncovering new potential targets to enhance cancer virotherapy.
Sat Dec 01 00:00:00 UTC 2012
HLA-Cw*0102-restricted HIV-1 p24 epitope variants can modulate the binding of the inhibitory KIR2DL2 receptor and primary NK cell function.
PLoS pathogens
Fadda L,Körner C,Kumar S,van Teijlingen NH,Piechocka-Trocha A,Carrington M,Altfeld M
12-9983 was used in Flow cytometry/Cell sorting to demonstrate that sequence variations in position 7 of this epitope observed frequently in naturally occurring HIV-1 sequences can modulate binding to KIR2DL2.
Tue Jan 29 00:00:00 UTC 2013
Development of a humanized HLA-A2.1/DP4 transgenic mouse model and the use of this model to map HLA-DP4-restricted epitopes of HBV envelope protein.
PloS one
Ru Z,Xiao W,Pajot A,Kou Z,Sun S,Maillere B,Zhao G,Ojcius DM,Lone YC,Zhou Y
12-9983 was used in Flow cytometry/Cell sorting to develop a model to facilitate the identification of novel HLA-A2- and HLA-DP4-restricted epitopes and vaccine development as well as the characterization of HLA-DP4-restricted responses against infection in humans.
Mon Jul 09 00:00:00 UTC 2012
Chlamydia trachomatis immune evasion via downregulation of MHC class I surface expression involves direct and indirect mechanisms.
Infectious diseases in obstetrics and gynecology
Ibana JA,Schust DJ,Sugimoto J,Nagamatsu T,Greene SJ,Quayle AJ
12-9983 was used in Flow cytometry/Cell sorting to demonstrate that major histocompatibility complex class I downregulation in the presence of Chlamydia trachomatis is mediated by direct and indirect (soluble) factors.
Fri Oct 28 00:00:00 UTC 2011
Cowpox virus inhibits the transporter associated with antigen processing to evade T cell recognition.
Cell host & microbe
Alzhanova D,Edwards DM,Hammarlund E,Scholz IG,Horst D,Wagner MJ,Upton C,Wiertz EJ,Slifka MK,Früh K
12-9983 was used in Flow cytometry/Cell sorting to identify cowpox virus proteins that mediate T cell evasion in multiple hosts.
Thu Nov 19 00:00:00 UTC 2009
Cowpox virus inhibits the transporter associated with antigen processing to evade T cell recognition.
Cell host & microbe
Alzhanova D,Edwards DM,Hammarlund E,Scholz IG,Horst D,Wagner MJ,Upton C,Wiertz EJ,Slifka MK,Früh K
12-9983 was used in Flow cytometry/Cell sorting to identify cowpox virus proteins that mediate T cell evasion in multiple hosts.
Thu Nov 19 00:00:00 UTC 2009
Construction of soluble Mamu-b*1703, a class I major histocompatibility complex of Chinese rhesus macaques, monomer and tetramer loaded with a simian immunodeficiency virus peptide.
Cellular & molecular immunology
Ouyang D,Wang X,He X,Xu L,Shi H,Gao Q,Guo H
12-9983 was used in an ELISA assay to investigate how some antigenic peptides reported in Indian-origin macaques could be directly recruited as ligands for construction of Chinese macaque MHC tetramers.
Wed Apr 01 00:00:00 UTC 2009
Sensitization of human breast cancer cells to natural killer cell-mediated cytotoxicity by proteasome inhibition.
Clinical and experimental immunology
Ames E,Hallett WH,Murphy WJ
12-9983 was used in Flow cytometry/Cell sorting to investigate whether bortezomib can sensitise human breast cancer cells to killing by the human NK cell line, NK-92.
Sun Mar 01 00:00:00 UTC 2009
Signaling of a varicelloviral factor across the endoplasmic reticulum membrane induces destruction of the peptide-loading complex and immune evasion.
The Journal of biological chemistry
Loch S,Klauschies F,Schölz C,Verweij MC,Wiertz EJ,Koch J,Tampé R
12-9983 was used in Flow cytometry/Cell sorting to show that a small viral factor and their signalling across endoplasmic reticulum membranes are essential for targeted degradation of multi-subunit membrane complexes.
Fri May 09 00:00:00 UTC 2008
Identification of a lysosomal peptide transport system induced during dendritic cell development.
The Journal of biological chemistry
Demirel O,Waibler Z,Kalinke U,Grünebach F,Appel S,Brossart P,Hasilik A,Tampé R,Abele R
12-9983 was used in Flow cytometry/Cell sorting to suggest a mechanism by which antigenic peptides have access to the lysosomal compartment in professional antigen-presenting cells.
Fri Dec 28 00:00:00 UTC 2007
High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss.
Human immunology
Koene GJ,Arts-Hilkes YH,van Dijk AJ,van der Ven KJ,Slootweg PJ,de Weger RA,Tilanus MG
Wed Dec 01 00:00:00 UTC 2004
A study of human leukocyte antigen G expression in hydatidiform moles.
American journal of obstetrics and gynecology
Goldman-Wohl D,Ariel I,Greenfield C,Hochner-Celnikier D,Lavy Y,Yagel S
Wed Aug 01 00:00:00 UTC 2001
