Invitrogen

HLA-ABC Monoclonal Antibody (W6/32)

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HLA-ABC Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MHC I HLA-A B C (green) showing staining in the cytoplasm and membrane of non-permeabilized Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an MHC I (HLA-A B C) monoclonal antibody (Product # MA5-11723) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

HLA-ABC Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Frozen human tonsil section stained with MHC-I antibody using UltraVision LP and AEC chromogen. Note membrane staining.

HLA-ABC Antibody in Western Blot (WB) — Western blot analysis of MHC I (HLA-A B C) was performed by loading 25 µg of HL60 (lane 1) and Raji (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a MHC I (HLA-A B C) monoclonal antibody (Product # MA5-11723) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~41 kDa.

HLA-ABC Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of MHC I in peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-11723) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 µL of cell buffer.

Product Details

MA5-11723

Applications	Tested Dilution	Publications
Western Blot (WB)	1:10-1:200	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	1:10-1:200	View 7 publications 7 publications
Flow Cytometry (Flow)	0.5 µg/test	View 3 publications 3 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	W6/32
Immunogen	Cell membranes of human tonsil lymphocytes
Conjugate	Unconjugated Biotin FITC Functional Grade
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10985125

Product Specific Information

MA5-11723 targets MHC I (HLA-A B C) in IHC (F), ICC/IF, WB and FACS applications and shows reactivity with Human and Mouse samples.

The MA5-11723 immunogen is cell membranes of human tonsil lymphocytes.

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MITF induces escape from innate immunity in melanoma.

Journal of experimental & clinical cancer research : CR

Sánchez-Del-Campo L,Martí-Díaz R,Montenegro MF,González-Guerrero R,Hernández-Caselles T,Martínez-Barba E,Piñero-Madrona A,Cabezas-Herrera J,Goding CR,Rodríguez-López JN

MA5-11723 was used in Immunocytochemistry to examine the potential role of the lineage survival oncogene MITF to modulate the anti-melanoma immune response.

Wed Mar 31 00:00:00 EDT 2021

Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival.

Aging cell

Chen LJ,Yang ZX,Wang Y,Du L,Li YR,Zhang NN,Gao WY,Peng RR,Zhu FY,Wang LL,Li CR,Li JM,Wang FQ,Sun QY,Zhang D

Published figure using HLA-ABC monoclonal antibody (Product # MA5-11723) in Western Blot

Sun Dec 01 00:00:00 EST 2019

Efficacy research of salazosulfamide in ankylosing spondylitis and NAT1 gene polymorphism.

Experimental and therapeutic medicine

Ma XF,Wang XD,Liu RR,Luan QX

MA5-11723 was used in Western Blotting to explore the correlation of salazosulfamide efficacy on ankylosing spondylitis and N-acetyltransferase 1 gene polymorphism.

Sun Oct 01 00:00:00 EDT 2017

B. abortus RNA is the component involved in the down-modulation of MHC-I expression on human monocytes via TLR8 and the EGFR pathway.

PLoS pathogens

Milillo MA,Velásquez LN,Trotta A,Delpino MV,Marinho FV,Balboa L,Vermeulen M,Espindola SL,Rodriguez-Rodrigues N,Fernández GC,Oliveira SC,Giambartolomei GH,Barrionuevo P

MA5-11723 was used in Immunocytochemistry-immunoflourescence to demonstrate that the down-modulation of MHC-I expression is not mediated by well-known Brucella virulence factors but instead by B. abortus RNA.

Tue Aug 01 00:00:00 EDT 2017

A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells.

Nature medicine

Reinisch A,Thomas D,Corces MR,Zhang X,Gratzinger D,Hong WJ,Schallmoser K,Strunk D,Majeti R

MA511723 was used in flow cytometry to develop and characterize a humanized ossicle xenotransplantation approach

Fri Jul 01 00:00:00 EDT 2016

Hepatitis C virus infection in inclusion body myositis: A case-control study.

Neurology

Uruha A,Noguchi S,Hayashi YK,Tsuburaya RS,Yonekawa T,Nonaka I,Nishino I

MA5-11723 was used in immunohistochemistry - paraffin section to determine if there is an association between inclusion body myositis and hepatitis C virus infection

Tue Jan 19 00:00:00 EST 2016

Expanded cytotoxic T-cell lymphocytes target the latent HIV reservoir.

The Journal of infectious diseases

Sung JA,Lam S,Garrido C,Archin N,Rooney CM,Bollard CM,Margolis DM

MA5-11723 was used in Immunocytochemistry to evaluate the use of expanded cytotoxic T-cell lymphocytes to target the latent HIV reservoir in infected individuals.

Wed Jul 15 00:00:00 EDT 2015

Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.

mBio

Olofsson A,Nygård Skalman L,Obi I,Lundmark R,Arnqvist A

MA5-11723 was used in immunocytochemistry to study the role of clathrin-dependent and clathrin-independent routes in the uptake of Helicobacter pylori vesicles by gastric epithelial cells

Tue May 20 00:00:00 EDT 2014

Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.

Anticancer research

Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK

MA5-11723 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells

Fri Nov 01 00:00:00 EDT 2013

SHP2 is overexpressed and inhibits pSTAT1-mediated APM component expression, T-cell attracting chemokine secretion, and CTL recognition in head and neck cancer cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Leibowitz MS,Srivastava RM,Andrade Filho PA,Egloff AM,Wang L,Seethala RR,Ferrone S,Ferris RL

MA5-11723 was used in an ELISpot assay to investigate whether an overexpressed phosphatase is responsible for basal suppression of activated STAT1 and subsequent antigen processing machinery component-mediated immune escape in head and neck cells.

Fri Feb 15 00:00:00 EST 2013

The human herpesvirus-7 (HHV-7) U21 immunoevasin subverts NK-mediated cytoxicity through modulation of MICA and MICB.

PLoS pathogens

Schneider CL,Hudson AW

MA5-11723 was used in western blot to report that the human herpesvirus-7 U21 gene product interferes with natural killer cell recognition

Tue Nov 01 00:00:00 EDT 2011

Induction of metastatic cancer stem cells from the NK/LAK-resistant floating, but not adherent, subset of the UP-LN1 carcinoma cell line by IFN-γ.

Laboratory investigation; a journal of technical methods and pathology

Chen HC,Chou AS,Liu YC,Hsieh CH,Kang CC,Pang ST,Yeh CT,Liu HP,Liao SK

MA5-11723 was used in flow cytometry to study the induction of metastatic cancer stem cells from UP-LN1 cell line

Sat Oct 01 00:00:00 EDT 2011

Alterations in the Arf6-regulated plasma membrane endosomal recycling pathway in cells overexpressing the tetraspan protein Gas3/PMP22.

Journal of cell science

Chies R,Nobbio L,Edomi P,Schenone A,Schneider C,Brancolini C

MA5-11723 was used in immunocytochemistry to study alterations in Arf6-regulated plasma membrane endosomal recycling in cells overexpressing Gas3/PMP22

Sat Mar 15 00:00:00 EST 2003

Establishment of a quantitative ELISA capable of determining peptide - MHC class I interaction.

Tissue antigens

Sylvester-Hvid C,Kristensen N,Blicher T,Ferré H,Lauemøller SL,Wolf XA,Lamberth K,Nissen MH,Pedersen LØ,Buus S

MA5-11723 was used in ELISA to develop a quantitative ELISA assay for studying peptide-MHC class I interactions

Mon Apr 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: A29 protein; alpha chain of MHC class II; antigen presenting molecule; class I MHC, HLA-B antigen; epididymis secretory protein Li 83; H-2K; H-2K(d); H2-K; histocompatibility antigen; HLA -B; HLA A; HLA B-40011; HLA B44; HLA C; HLA class I antigen; HLA class I antigen HLA-B; HLA class I histocompatibility antigen C alpha chain; HLA class I histocompatibility antigen Cw-1 alpha chain; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, B-47 alpha chain; HLA class I histocompatibility antigen, B-50 alpha chain; HLA class I histocompatibility antigen, B-82 alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA class I histocompatibility antigen, Cw-15 alpha chain; HLA class I histocompatibility antigen, Cw-18 alpha chain; HLA locus; HLA molecule; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-Aw33.1; HLA-B; HLA-B 1502; HLA-B alpha chain; HLA-B alpha chain (B*2706); HLA-B alpha chain (B*5002); HLA-B alpha-chain; HLA-B35; HLA-B35 antigen; HLA-Bw50 antigen; HLA-C; HLA-C alpha chain; HLA-C alpha chain (HLA-Cw*15v); HLA-C alpha chain for Cw*18GB; HLA-C alpha-chain; HLA-C antigen; HLA-Cw; HLA-Cw*04GB; HLA-Cw*1702; HLA-Cw*18GB; HLA-Cw-1503; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; hypothetical protein; lecocyte antigen B; leucocyte antigen A; Leukocyte Antigen; leukocyte antigen C; leukocyte antigen class I-A; leukocyte antigen class I-B; Leukoocyte Antigen; lymphocyte antigen; major histocompatability complex B antigen; major histocompatibility antigen HLA-C; MCH class I antigen; MGC184092; MGC7052; MHC 1; MHC antigen; MHC clas I antigen; MHC Class 1; MHC class 1 antigen; MHC class I; MHC class I alpha chain; MHC class I anitgen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen B*47; MHC class I antigen B*50; MHC class I antigen B*82; MHC class I antigen Cw*15; MHC class I antigen Cw*18; MHC class I antigen GN00104; MHC class I antigen heavy chain; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen null protein; MHC class I antigen SHCHA; MHC class I antigene; MHC class I histocompatibility antigen; MHC class I human leukocyte antigen; MHC class I molecule; MHC class I protein; MHC class II antigen; MHC class l antigen; MHC classI antigen; MHC HLA-ABC; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B27-HS; MHC HLA-B27d; MHC HLA-B39N; MHC I HLA; MHC1; surface antigen; truncated class I antigen; truncated MHC class I antigen

Gene Aliases: AS; Aw-33; Aw-74; B-4901; B-5001; Bw-47; Bw-50; D6S204; HEL-S-83; HLA-A; HLA-A11; HLA-A33; HLA-B; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HLA-C; HLA-Cw; HLA-DQB1; HLA-DRB1; HLA-JY3; HLAA; HLAB; HLAC; HLC-C; MHC; PSORS1; SPDA1

UniProt ID: (Human) P04439, (Human) P01889, (Human) Q29959

Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107

Molecular Function: major histocompatibility complex protein