Log fluorescence intensity profiles of human PBLs gated on lymphocytes and analyzed on a BD LSR II flow cytometer (BD Biosciences, San Jose, CA) using 405 nm excitation and the specified emission filters. The data files were analyzed using FlowJo™ software (Treestar, Inc., www.flowjo.com). The black line represents cells stained with anti-human HLA-DR antibody conjugate and the gray line represents unstained cells. Note: Flow cytometric data shown may not necessarily have been generated using the enclosed lot of reagent. For this reason, and due to differences in flow cytometers and cytometer settings, results may vary from those illustrated above. We recommend titrating reagents to determine optimal conditions for use in your systems.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
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