|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Murine MHC Class II antigen.|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40077 detects MHC II from mouse samples, and crossreacts with human MHC class II on tonsil.
MA1-40077 has been successfully used in immunohistochemistry (frozen), immunohistochemistry (paraffin), and flow cytometry applications.
The MA1-40077 immunogen is mouse MHC Class II antigen.
The monoclonal antibody ER-TR3 reacts with mouse major histocompatibility complex class II (MHC class II), also know as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.