Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis.<br />Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6). Blocking antibodies: Column 1-2: none Column 3: Isotype mouse IgG2a control Column 4: anti-human HLA-G (87G) Purified Column 5: anti-human HLA-G (87G) F(ab180)2 fragment Column 6: anti-human HLA-G (MEM-G/9)
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 ug/ml|
|Flow Cytometry (Flow)||2 ug/ml|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). It will not cross-react with mouse or rat.
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.