Invitrogen

HLA-G Monoclonal Antibody (87G), eBioscience™

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HLA-G Antibody in Flow Cytometry (Flow) — Staining of stimulated U937 cells with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (blue) or 0.25 µg of Anti-Human HLA-G Purified (purple) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012).Total viable cells were used for analysis.

HLA-G Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — FIGURE 2 Identification of TGF-beta1-producing cells at the maternal-foetal interface in normal pregnancy at gestational weeks 7-9. A-C, In situ hybridization of TGF-beta1 (blue) and immunohistochemistry staining of HLA-G (yellow in A and B) or CD31 (yellow in C) in normal pregnant decidua. Middle panels are enlargement of the rectangular areas in left panels, showing the enEVTs in remodelled SPA (A), iEVTs in the area nearby the remodelled SPA (B) and dECs in unremodelled SPA (C). Right panels are negative control of in situ hybridization, with the immunohistochemistry signals of HLA-G (A, B) or CD31 (C). D, E, Immunofluorescence of HLA-G (red) and NCAM1 (green) in remodelled SPA (D) and the area nearby remodelled SPA (E). F, Flow cytometry of TGF-beta1 expression in EVTs of normal pregnancy. Left panel, FACS isolation of enEVTs and iEVTs with antibodies against HLA-G and NCAM1. Middle panel, flow cytometry of TGF-beta1-positive enEVTs that are gated from the left panel as HLA-G + NCAM1 + . Right panel: flow cytometry analysis of TGF-beta1-positive iEVTs that were gated from the left panel as HLA-G + NCAM1 - . G, FACS isolation of CD31 + NCAM1 - dECs in normal pregnancy (left panel), and flow cytometry of TGF-beta1-positive dECs gated from the left panel as CD31 + NCAM1 - (right panel). H, The statistical analysis of TGF-beta1-positive primary cells based on the results from 5 normal pregnant cases. I, ELISA for TGF-beta1 in supernatants of the FACS-sorted enEVTs, iEVTs and

Product Details

14-9957-82

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Flow Cytometry (Flow)	0.5 µg/test	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	87G
Conjugate	Unconjugated APC PE PerCP-eFluor 710
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_1210776

Product Specific Information

Description: The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintenance of transplanted organs.

Applications Reported: This 87G antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested: This 87G antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

Published figure using HLA-G monoclonal antibody (Product # 14-9957-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Placental endovascular extravillous trophoblasts (enEVTs) educate maternal T-cell differentiation along the maternal-placental circulation.

Cell proliferation

Ma Y,Yang Q,Fan M,Zhang L,Gu Y,Jia W,Li Z,Wang F,Li YX,Wang J,Li R,Shao X,Wang YL

Published figure using HLA-G monoclonal antibody (Product # 14-9957-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

The Killer Cell Ig-like Receptor 2DL4 Expression in Human Mast Cells and Its Potential Role in Breast Cancer Invasion.

Cancer immunology research

Ueshima C,Kataoka TR,Hirata M,Furuhata A,Suzuki E,Toi M,Tsuruyama T,Okayama Y,Haga H

14-9957 was used in Immunohistochemistry to show that KIR2DL4 is expressed in human nonneoplastic mast cells and enhances the invasive activity of HLA-G+ cancer cells.

Sat Aug 01 00:00:00 EDT 2015

L3MBTL1 deficiency directs the differentiation of human embryonic stem cells toward trophectoderm.

Stem cells and development

Hoya-Arias R,Tomishima M,Perna F,Voza F,Nimer SD

Published figure using HLA-G monoclonal antibody (Product # 14-9957-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G

Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G

UniProt ID: (Human) P17693

Entrez Gene ID: (Human) 3135

Molecular Function: major histocompatibility complex protein

Function(s)

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent positive regulation of tolerance induction positive regulation of T cell tolerance induction immune response-inhibiting cell surface receptor signaling pathway cellular defense response antigen processing and presentation positive regulation of interleukin-12 production negative regulation of T cell proliferation positive regulation of regulatory T cell differentiation regulation of immune response negative regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway negative regulation of dendritic cell differentiation

Process(es)

antigen binding receptor binding peptide antigen binding protein homodimerization activity

HLA-G Monoclonal Antibody (87G), eBioscience™

Product Details

14-9957-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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