|ELISA (ELISA)||1 µg/mL|
|Flow Cytometry (Flow)||2 µg/mL|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/mL|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||4° C, do not freeze|
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). It will not cross-react with mouse or rat.
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha chain G; b2 microglobulin; class I; DADB-15K14.8; G; HLA class I histocompatibility antigen; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; MHC-G
Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G
UniProt ID: (Human) P17693
Entrez Gene ID: (Human) 3135
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