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Product Details
MA1-10357
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
Assay-dependent | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
10 µg/mL | - |
Immunohistochemistry (Frozen) (IHC (F)) |
10 µg/mL | - |
Immunocytochemistry (ICC/IF) |
Assay-dependent | - |
Flow Cytometry (Flow) |
1-4 µg/mL | - |
ELISA (ELISA) |
Assay-dependent | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
5A6G7 |
Immunogen |
C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin. |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4 |
Contains |
15mM sodium azide |
Storage conditions |
4° C, do not freeze |
RRID |
AB_11157238 |
Product Specific Information
This antibody does not cross-react with the full-length HLA-G1 isoform and therefore is able to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Purity is >95% by SDS-PAGE.
ELISA Positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (Product # MA1-19526).
Western blot Positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE.
Western Blot: Reducing conditions.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G
Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G
UniProt ID: (Human) P17693
Entrez Gene ID: (Human) 3135
Molecular Function:
major histocompatibility complex protein
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent
positive regulation of tolerance induction
positive regulation of T cell tolerance induction
immune response-inhibiting cell surface receptor signaling pathway
cellular defense response
antigen processing and presentation
positive regulation of interleukin-12 production
negative regulation of T cell proliferation
positive regulation of regulatory T cell differentiation
regulation of immune response
negative regulation of immune response
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
negative regulation of dendritic cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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