Bethyl Laboratories

HLX Polyclonal Antibody

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HLX Antibody in Western Blot (WB) — **Detection of human HLX by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from 293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-HLX antibody A303-584A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HLX was also immunoprecipitated by rabbit anti-HLX antibody BL12796, which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Product Details

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

2-10 µg/mg lysate

Western Blot (WB)

1:2,000-1:10,000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 375 and 425

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

HLX is a transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H2.0-like homeo box 1; H2.0-like homeo box-1; H2.0-like homeobox 1; H2.0-like homeobox protein; Homeobox protein HB24; Homeobox protein HLX1

Gene Aliases: HB24; HLX; HLX1

UniProt ID: (Human) Q14774

Entrez Gene ID: (Human) 3142

Function(s)