Bethyl Laboratories

HLX Polyclonal Antibody

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HLX Antibody in Western Blot (WB) — **Detection of human HLX by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from 293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-HLX antibody A303-584A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HLX was also immunoprecipitated by rabbit anti-HLX antibody BL12796, which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Product Details

A303-584A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunoprecipitation (IP)	2-10 µg/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 375 and 425 View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

HLX is a transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: H2.0-like homeo box 1; H2.0-like homeo box-1; H2.0-like homeobox 1; H2.0-like homeobox protein; Homeobox protein HB24; Homeobox protein HLX1

Gene Aliases: HLX; HLX1