|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 2 and 159 of HMGB1|
|Storage buffer||PBS, pH 7|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17277 targets HMGB1 in IHC (P) and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The MA5-17277 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 2 and 159 of HMGB1.
HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.