Western blot analysis of HMGB1 in (lane 1) mouse kidney (lane 2) HeLa (lane 3) HeLa nuclear (lane 4) Jurkat (lane 5) HEK-293T (lane 6) MDCK (lane 7) PC12 (lane 8) NIH-3T3 (lane 9) P19 lysates. Samples were separated on SDS-PAGE and probed with a HMGB1 monoclonal antibody (Product # MA1-20338) at a dilution of 1 µg/ml. The antibody was developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
|Tested species reactivity||Rat, Dog, Chicken, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length, human HMGB1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20338 detects HMGB1 in human, mouse, chicken, rat, and canine samples.
MA1-20338 has been successfully used in ELISA and Western blot procedures.
The MA1-20338 immunogen is recombinant, full length, human HMGB1 protein.
HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.